ID   CBIJ_SYNY3              Reviewed;         261 AA.
AC   P72711;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Cobalt-precorrin-6A reductase;
DE            EC=1.3.1.106;
GN   Name=cbiJ; Synonyms=cobK; OrderedLocusNames=slr0252;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
CC   -!- FUNCTION: Catalyzes the reduction of the macrocycle of cobalt-
CC       precorrin-6A to cobalt-precorrin-6B. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Co-precorrin-6B + NAD(+) = Co-precorrin-6A + H(+) + NADH;
CC         Xref=Rhea:RHEA:15625, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:60064, ChEBI:CHEBI:72780;
CC         EC=1.3.1.106;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route):
CC       step 7/10.
CC   -!- SIMILARITY: Belongs to the precorrin-6x reductase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00356}.
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DR   EMBL; BA000022; BAA16718.1; -; Genomic_DNA.
DR   PIR; S74566; S74566.
DR   AlphaFoldDB; P72711; -.
DR   SMR; P72711; -.
DR   IntAct; P72711; 2.
DR   STRING; 1148.1651791; -.
DR   PaxDb; P72711; -.
DR   EnsemblBacteria; BAA16718; BAA16718; BAA16718.
DR   KEGG; syn:slr0252; -.
DR   eggNOG; COG2099; Bacteria.
DR   InParanoid; P72711; -.
DR   OMA; THPYAAQ; -.
DR   PhylomeDB; P72711; -.
DR   UniPathway; UPA00148; UER00228.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0016994; F:precorrin-6A reductase activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR003723; Precorrin-6x_reduct.
DR   PANTHER; PTHR36925; PTHR36925; 1.
DR   Pfam; PF02571; CbiJ; 1.
DR   TIGRFAMs; TIGR00715; precor6x_red; 1.
DR   PROSITE; PS51014; COBK_CBIJ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..261
FT                   /note="Cobalt-precorrin-6A reductase"
FT                   /id="PRO_0000135916"
SQ   SEQUENCE   261 AA;  28565 MW;  4F59669CF62611BE CRC64;
     MVDSLATVWL IGGTVDSRAV AEGLIAQGIN CLVTVTTSEA KHLYPIHQCL TVHVGALTPQ
     EIPKFLKRHS IAVIVDASHP FAAQITTTVT AIAKEQQIPY IRFERPPLAL GKNTLEVPDI
     QSLTRGKYQP YLRGKRVLLT VGARWLSHFS LLQDEAVLFA RILPYPQALA QAIAAGFTSD
     RIIALRPPVA EPLEKALWQQ WQIQGVVTKA SGAQGGELVK QKVAEALGVN LIRIARPQTI
     PGQITDDLSQ INQFCQRHLP S