CBIL_SALTY
ID CBIL_SALTY Reviewed; 237 AA.
AC Q05593;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Cobalt-precorrin-2 C(20)-methyltransferase;
DE EC=2.1.1.151;
DE AltName: Full=S-adenosyl-L-methionine--cobalt-precorrin-2 methyltransferase;
GN Name=cbiL; OrderedLocusNames=STM2024;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF N-TERMINUS.
RC STRAIN=LT2;
RX PubMed=8501034; DOI=10.1128/jb.175.11.3303-3316.1993;
RA Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M.;
RT "Characterization of the cobalamin (vitamin B12) biosynthetic genes of
RT Salmonella typhimurium.";
RL J. Bacteriol. 175:3303-3316(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP PROTEIN SEQUENCE OF 1-10.
RX PubMed=1451790; DOI=10.1016/0014-5793(92)80213-z;
RA Roessner C.A., Warren M.J., Santander P.J., Atshaves B.P., Ozaki S.,
RA Stolowich N.J., Iida K., Scott A.I.;
RT "Expression of 9 Salmonella typhimurium enzymes for cobinamide synthesis.
RT Identification of the 11-methyl and 20-methyl transferases of corrin
RT biosynthesis.";
RL FEBS Lett. 301:73-78(1992).
CC -!- FUNCTION: Methylates cobalt-precorrin-2 at the C-20 position to produce
CC cobalt-precorrin-3A in the anaerobic cobalamin biosynthesis pathway.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Co-precorrin-2 + S-adenosyl-L-methionine = Co-precorrin-3 +
CC H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:17997,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:60053, ChEBI:CHEBI:60060; EC=2.1.1.151;
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route):
CC step 2/10.
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC {ECO:0000305}.
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DR EMBL; L12006; AAA27263.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL20928.1; -; Genomic_DNA.
DR RefSeq; NP_460969.1; NC_003197.2.
DR RefSeq; WP_001013552.1; NC_003197.2.
DR AlphaFoldDB; Q05593; -.
DR SMR; Q05593; -.
DR STRING; 99287.STM2024; -.
DR PaxDb; Q05593; -.
DR EnsemblBacteria; AAL20928; AAL20928; STM2024.
DR GeneID; 1253545; -.
DR KEGG; stm:STM2024; -.
DR PATRIC; fig|99287.12.peg.2146; -.
DR HOGENOM; CLU_076014_3_0_6; -.
DR OMA; MEYPVTT; -.
DR PhylomeDB; Q05593; -.
DR BioCyc; MetaCyc:MON-13216; -.
DR BioCyc; SENT99287:STM2024-MON; -.
DR UniPathway; UPA00148; UER00224.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0043781; F:cobalt-factor II C20-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0030788; F:precorrin-2 C20-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd11645; Precorrin_2_C20_MT; 1.
DR Gene3D; 3.30.950.10; -; 1.
DR Gene3D; 3.40.1010.10; -; 1.
DR InterPro; IPR000878; 4pyrrol_Mease.
DR InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR InterPro; IPR014776; 4pyrrole_Mease_sub2.
DR InterPro; IPR012382; CobI/CbiL.
DR InterPro; IPR006364; CobI/CbiL/CobIJ_dom.
DR InterPro; IPR003043; Uropor_MeTrfase_CS.
DR Pfam; PF00590; TP_methylase; 1.
DR PIRSF; PIRSF036427; Precrrn-2_mtase; 1.
DR SUPFAM; SSF53790; SSF53790; 1.
DR TIGRFAMs; TIGR01467; cobI_cbiL; 1.
DR PROSITE; PS00839; SUMT_1; 1.
DR PROSITE; PS00840; SUMT_2; 1.
PE 1: Evidence at protein level;
KW Cobalamin biosynthesis; Direct protein sequencing; Methyltransferase;
KW Reference proteome; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..237
FT /note="Cobalt-precorrin-2 C(20)-methyltransferase"
FT /id="PRO_0000150388"
FT CONFLICT 188
FT /note="Q -> T (in Ref. 1; AAA27263)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 237 AA; 25806 MW; 3D7215BDC67D2067 CRC64;
MNGKLYALST GPGAPDLITV RAARILGSLD ILYAPAGRKG GDSLALSIVR DYLGEQTEVR
CCHFPMSADG AEKEAVWNEV AAALTAEVEA GKQVGFITLG DAMLFSTWIF LLQRIGCPEW
LEIVPGVTSF AAIAARAKMP LAIERQSLAV ISCTAPEAEI AQALQQHDSL VLMKVYGRFA
RIKALLAQAG LLECALMMSE ATLPGEQCWR HLHEVNDDRP LPYFSTILVN KQWEYAE
