ID   CBIM2_METPE             Reviewed;         235 AA.
AC   B8GEB7;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Putative cobalt transport protein CbiM 2 {ECO:0000255|HAMAP-Rule:MF_01462};
DE   AltName: Full=Energy-coupling factor transporter probable substrate-capture protein CbiM 2 {ECO:0000255|HAMAP-Rule:MF_01462};
DE            Short=ECF transporter S component CbiM 2 {ECO:0000255|HAMAP-Rule:MF_01462};
GN   Name=cbiM2 {ECO:0000255|HAMAP-Rule:MF_01462}; OrderedLocusNames=Mpal_2333;
OS   Methanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanomicrobiales; Methanoregulaceae; Methanosphaerula.
OX   NCBI_TaxID=521011;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1556 / DSM 19958 / E1-9c;
RX   PubMed=26543115; DOI=10.1128/genomea.01280-15;
RA   Cadillo-Quiroz H., Browne P., Kyrpides N., Woyke T., Goodwin L., Detter C.,
RA   Yavitt J.B., Zinder S.H.;
RT   "Complete Genome Sequence of Methanosphaerula palustris E1-9CT, a
RT   Hydrogenotrophic Methanogen Isolated from a Minerotrophic Fen Peatland.";
RL   Genome Announc. 3:0-0(2015).
CC   -!- FUNCTION: Part of the energy-coupling factor (ECF) transporter complex
CC       CbiMNOQ involved in cobalt import. {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- SUBUNIT: Forms an energy-coupling factor (ECF) transporter complex
CC       composed of an ATP-binding protein (A component, CbiO), a transmembrane
CC       protein (T component, CbiQ) and 2 possible substrate-capture proteins
CC       (S components, CbiM and CbiN) of unknown stoichimetry.
CC       {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01462};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- SIMILARITY: Belongs to the CbiM family. {ECO:0000255|HAMAP-
CC       Rule:MF_01462}.
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DR   EMBL; CP001338; ACL17618.1; -; Genomic_DNA.
DR   RefSeq; WP_012618937.1; NC_011832.1.
DR   AlphaFoldDB; B8GEB7; -.
DR   SMR; B8GEB7; -.
DR   STRING; 521011.Mpal_2333; -.
DR   EnsemblBacteria; ACL17618; ACL17618; Mpal_2333.
DR   GeneID; 7272054; -.
DR   KEGG; mpl:Mpal_2333; -.
DR   eggNOG; arCOG02248; Archaea.
DR   HOGENOM; CLU_052508_3_0_2; -.
DR   OMA; IAWGVIC; -.
DR   OrthoDB; 94360at2157; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000002457; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0015087; F:cobalt ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_01462; CbiM; 1.
DR   InterPro; IPR018024; CbiM.
DR   InterPro; IPR002751; CbiM/NikMN.
DR   PANTHER; PTHR43627; PTHR43627; 1.
DR   Pfam; PF01891; CbiM; 1.
DR   TIGRFAMs; TIGR00123; cbiM; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cobalamin biosynthesis; Cobalt; Cobalt transport;
KW   Ion transport; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..235
FT                   /note="Putative cobalt transport protein CbiM 2"
FT                   /id="PRO_0000411162"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        80..100
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        107..127
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        135..155
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        160..180
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        181..201
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
SQ   SEQUENCE   235 AA;  25294 MW;  4BF68A3AEF8CB116 CRC64;
     MHIMEGFLPA GWCLVWWLIA LPFLVMGIIQ LRRMMKEDRE YLPLLGVCGA FIFILSALKL
     PSVTGSCSHP TGTGLSTICF GYCVTAVVGA IVLLFQALLL AHGGLSTMGA NMVSMAIGGP
     IAGYAVYKLM KDTSINIYVT VFLASAVADI VTYIITSFEL ALAYPAQVGG FLASFSAFFS
     IFAITQIPLS IMEGVVLALV FKYIIQLKPE IILKLHVFSE EQIAKARLAG DAEVA