ID   CBIM_ANOFW              Reviewed;         250 AA.
AC   B7GLU2;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Cobalt transport protein CbiM {ECO:0000255|HAMAP-Rule:MF_01462};
DE   AltName: Full=Energy-coupling factor transporter probable substrate-capture protein CbiM {ECO:0000255|HAMAP-Rule:MF_01462};
DE            Short=ECF transporter S component CbiM {ECO:0000255|HAMAP-Rule:MF_01462};
DE   Flags: Precursor;
GN   Name=cbiM {ECO:0000255|HAMAP-Rule:MF_01462}; OrderedLocusNames=Aflv_2184;
OS   Anoxybacillus flavithermus (strain DSM 21510 / WK1).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Anoxybacillus.
OX   NCBI_TaxID=491915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 21510 / WK1;
RX   PubMed=19014707; DOI=10.1186/gb-2008-9-11-r161;
RA   Saw J.H., Mountain B.W., Feng L., Omelchenko M.V., Hou S., Saito J.A.,
RA   Stott M.B., Li D., Zhao G., Wu J., Galperin M.Y., Koonin E.V.,
RA   Makarova K.S., Wolf Y.I., Rigden D.J., Dunfield P.F., Wang L., Alam M.;
RT   "Encapsulated in silica: genome, proteome and physiology of the
RT   thermophilic bacterium Anoxybacillus flavithermus WK1.";
RL   Genome Biol. 9:R161.1-R161.16(2008).
CC   -!- FUNCTION: Part of the energy-coupling factor (ECF) transporter complex
CC       CbiMNOQ involved in cobalt import. {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- SUBUNIT: Forms an energy-coupling factor (ECF) transporter complex
CC       composed of an ATP-binding protein (A component, CbiO), a transmembrane
CC       protein (T component, CbiQ) and 2 possible substrate-capture proteins
CC       (S components, CbiM and CbiN) of unknown stoichimetry.
CC       {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01462};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- SIMILARITY: Belongs to the CbiM family. {ECO:0000255|HAMAP-
CC       Rule:MF_01462}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000922; ACJ34543.1; -; Genomic_DNA.
DR   RefSeq; WP_012575721.1; NC_011567.1.
DR   AlphaFoldDB; B7GLU2; -.
DR   SMR; B7GLU2; -.
DR   STRING; 491915.Aflv_2184; -.
DR   EnsemblBacteria; ACJ34543; ACJ34543; Aflv_2184.
DR   KEGG; afl:Aflv_2184; -.
DR   PATRIC; fig|491915.6.peg.2243; -.
DR   eggNOG; COG0310; Bacteria.
DR   HOGENOM; CLU_052508_3_0_9; -.
DR   OMA; ANVFSMG; -.
DR   OrthoDB; 1632785at2; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000000742; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0015087; F:cobalt ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_01462; CbiM; 1.
DR   InterPro; IPR018024; CbiM.
DR   InterPro; IPR002751; CbiM/NikMN.
DR   PANTHER; PTHR43627; PTHR43627; 1.
DR   Pfam; PF01891; CbiM; 1.
DR   TIGRFAMs; TIGR00123; cbiM; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cobalamin biosynthesis; Cobalt; Cobalt transport;
KW   Ion transport; Membrane; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix; Transport.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   CHAIN           28..250
FT                   /note="Cobalt transport protein CbiM"
FT                   /id="PRO_0000411135"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        70..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        134..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        168..188
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        208..228
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
SQ   SEQUENCE   250 AA;  27150 MW;  1525F1D50AA42E86 CRC64;
     MKKPLFFIAS ACVTIYILFA LSPSVYAMHI MEGFLPWQWA LVWWLLFLPF FLVGMRNVAR
     LMRQRPEVKL LLALATAFTF VLSALKIPSV TGSSSHPTGT GLGALLFGPF VMTVIGTAVL
     LFQALLLAHG GVTTLGANAF SMAVVGPLVA YVLFSLCKKF GVSTRVSVFL AAMMADLATY
     VMTSIQLALA FPDATSGVWG AFLKFASIFA VTQIPLAITE GLLTVVVWNF LHTYSKRELT
     ILQQKGATIE