ID   CBIM_CLODR              Reviewed;         250 AA.
AC   C9YID6;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   24-NOV-2009, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Cobalt transport protein CbiM {ECO:0000255|HAMAP-Rule:MF_01462};
DE   AltName: Full=Energy-coupling factor transporter probable substrate-capture protein CbiM {ECO:0000255|HAMAP-Rule:MF_01462};
DE            Short=ECF transporter S component CbiM {ECO:0000255|HAMAP-Rule:MF_01462};
DE   Flags: Precursor;
GN   Name=cbiM {ECO:0000255|HAMAP-Rule:MF_01462};
GN   OrderedLocusNames=CDR20291_0329;
OS   Clostridioides difficile (strain R20291) (Peptoclostridium difficile).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC   Clostridioides.
OX   NCBI_TaxID=645463;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=R20291;
RX   PubMed=19781061; DOI=10.1186/gb-2009-10-9-r102;
RA   Stabler R.A., He M., Dawson L., Martin M., Valiente E., Corton C.,
RA   Lawley T.D., Sebaihia M., Quail M.A., Rose G., Gerding D.N., Gibert M.,
RA   Popoff M.R., Parkhill J., Dougan G., Wren B.W.;
RT   "Comparative genome and phenotypic analysis of Clostridium difficile 027
RT   strains provides insight into the evolution of a hypervirulent bacterium.";
RL   Genome Biol. 10:R102.1-R102.15(2009).
CC   -!- FUNCTION: Part of the energy-coupling factor (ECF) transporter complex
CC       CbiMNOQ involved in cobalt import. {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- SUBUNIT: Forms an energy-coupling factor (ECF) transporter complex
CC       composed of an ATP-binding protein (A component, CbiO), a transmembrane
CC       protein (T component, CbiQ) and 2 possible substrate-capture proteins
CC       (S components, CbiM and CbiN) of unknown stoichimetry.
CC       {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01462};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- SIMILARITY: Belongs to the CbiM family. {ECO:0000255|HAMAP-
CC       Rule:MF_01462}.
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DR   EMBL; FN545816; CBE02032.1; -; Genomic_DNA.
DR   RefSeq; WP_009888182.1; NC_013316.1.
DR   AlphaFoldDB; C9YID6; -.
DR   SMR; C9YID6; -.
DR   EnsemblBacteria; CBE02032; CBE02032; CDR20291_0329.
DR   KEGG; cdl:CDR20291_0329; -.
DR   HOGENOM; CLU_052508_3_0_9; -.
DR   OMA; ANVFSMG; -.
DR   UniPathway; UPA00148; -.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0015087; F:cobalt ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_01462; CbiM; 1.
DR   InterPro; IPR018024; CbiM.
DR   InterPro; IPR002751; CbiM/NikMN.
DR   PANTHER; PTHR43627; PTHR43627; 1.
DR   Pfam; PF01891; CbiM; 1.
DR   TIGRFAMs; TIGR00123; cbiM; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cobalamin biosynthesis; Cobalt; Cobalt transport;
KW   Ion transport; Membrane; Signal; Transmembrane; Transmembrane helix;
KW   Transport.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   CHAIN           26..250
FT                   /note="Cobalt transport protein CbiM"
FT                   /id="PRO_0000411138"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        132..152
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        163..183
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        205..225
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
SQ   SEQUENCE   250 AA;  26381 MW;  8A0645B42F655DC2 CRC64;
     MKQNIKLGVI AALMLIVLTP VTSNAMHIME GYLPVKWSIA WGVIFIPFFL VGLKSIGKIV
     KQDPKKKVLL ALCGAFVFVL SALKIPSVTG SCSHPTGVGL GAIMFGPSVM FVLGTIVLIF
     QALLLAHGGI TTLGANAFSM AIIGPIISFL IFKALKKKDG NNAMPVFLAA AIGDLATYTV
     TSIQLALAFP DPSGGVMASA IKFLGIFFMT QIPIAIAEGI LTVIVYNLIT ENGEKSILEN
     NDKGVKANEC