ID   CBIM_CLOTE              Reviewed;         241 AA.
AC   Q897L1;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Cobalt transport protein CbiM {ECO:0000255|HAMAP-Rule:MF_01462};
DE   AltName: Full=Energy-coupling factor transporter probable substrate-capture protein CbiM {ECO:0000255|HAMAP-Rule:MF_01462};
DE            Short=ECF transporter S component CbiM {ECO:0000255|HAMAP-Rule:MF_01462};
DE   Flags: Precursor;
GN   Name=cbiM {ECO:0000255|HAMAP-Rule:MF_01462}; OrderedLocusNames=CTC_00723;
OS   Clostridium tetani (strain Massachusetts / E88).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=212717;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Massachusetts / E88;
RX   PubMed=12552129; DOI=10.1073/pnas.0335853100;
RA   Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H.,
RA   Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A.,
RA   Gottschalk G.;
RT   "The genome sequence of Clostridium tetani, the causative agent of tetanus
RT   disease.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003).
CC   -!- FUNCTION: Part of the energy-coupling factor (ECF) transporter complex
CC       CbiMNOQ involved in cobalt import. {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- SUBUNIT: Forms an energy-coupling factor (ECF) transporter complex
CC       composed of an ATP-binding protein (A component, CbiO), a transmembrane
CC       protein (T component, CbiQ) and 2 possible substrate-capture proteins
CC       (S components, CbiM and CbiN) of unknown stoichimetry.
CC       {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01462};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01462}.
CC   -!- SIMILARITY: Belongs to the CbiM family. {ECO:0000255|HAMAP-
CC       Rule:MF_01462}.
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DR   EMBL; AE015927; AAO35325.1; -; Genomic_DNA.
DR   RefSeq; WP_035110300.1; NC_004557.1.
DR   AlphaFoldDB; Q897L1; -.
DR   SMR; Q897L1; -.
DR   STRING; 212717.CTC_00723; -.
DR   EnsemblBacteria; AAO35325; AAO35325; CTC_00723.
DR   GeneID; 64180510; -.
DR   KEGG; ctc:CTC_00723; -.
DR   HOGENOM; CLU_052508_3_0_9; -.
DR   OMA; ANVFSMG; -.
DR   OrthoDB; 1632785at2; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000001412; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0015087; F:cobalt ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_01462; CbiM; 1.
DR   InterPro; IPR018024; CbiM.
DR   InterPro; IPR002751; CbiM/NikMN.
DR   PANTHER; PTHR43627; PTHR43627; 1.
DR   Pfam; PF01891; CbiM; 1.
DR   TIGRFAMs; TIGR00123; cbiM; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cobalamin biosynthesis; Cobalt; Cobalt transport;
KW   Ion transport; Membrane; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix; Transport.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   CHAIN           24..241
FT                   /note="Cobalt transport protein CbiM"
FT                   /id="PRO_0000411140"
FT   TRANSMEM        29..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        121..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        160..180
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
FT   TRANSMEM        202..222
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01462"
SQ   SEQUENCE   241 AA;  26088 MW;  6A5E277159326877 CRC64;
     MKKNLTFFMV IALLFTITPN VYAMHIAEGF LPPMWSGVYF VISAPFIIIG LKQIRERAKD
     NKDIKMLLGL VAAYAFILSA MKIPSVTGSC SHPTGTGLSA IIFGPFISAI VGLIVLIFQA
     ILLAHGGITT LGANTLSMGI MGPIVSYLIY RGFKNKNQKV AVFLAATLGD LFTYFITSVQ
     LALAFPAQQG GIAASFAKFF SIFSITQIPL AIMEGILTVI IFEFVMKYAS KEIEVLGGVR
     K