ID   YJIK_ECOL6              Reviewed;         286 AA.
AC   Q8FA95;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 2.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Uncharacterized protein YjiK;
GN   Name=yjiK; OrderedLocusNames=c5415;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS) OF 35-286 IN COMPLEX WITH CALCIUM,
RP   AND COFACTOR.
RA   Stein A., Chhor G., Nocek B., Fenske R.J., Clancy S., Joachimiak A.;
RT   "Crystal structure of the C-terminal domain of the yjiK protein from
RT   Escherichia coli CFT073.";
RL   Submitted (FEB-2011) to the PDB data bank.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000305|Ref.2};
CC       Note=Calcium 4 is only bound to 1 residue in this protein.
CC       {ECO:0000305|Ref.2};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}; Single-pass membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the YjiK family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN83835.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE014075; AAN83835.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_001300028.1; NC_004431.1.
DR   PDB; 3QQZ; X-ray; 2.55 A; A=35-286.
DR   PDBsum; 3QQZ; -.
DR   AlphaFoldDB; Q8FA95; -.
DR   SMR; Q8FA95; -.
DR   STRING; 199310.c5415; -.
DR   DNASU; 1037188; -.
DR   EnsemblBacteria; AAN83835; AAN83835; c5415.
DR   KEGG; ecc:c5415; -.
DR   eggNOG; COG3204; Bacteria.
DR   HOGENOM; CLU_055438_1_0_6; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd09971; SdiA-regulated; 1.
DR   Gene3D; 2.120.10.30; -; 1.
DR   InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR   InterPro; IPR009722; SdiA-regulated.
DR   Pfam; PF06977; SdiA-regulated; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Calcium; Cell membrane; Membrane; Metal-binding;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..286
FT                   /note="Uncharacterized protein YjiK"
FT                   /id="PRO_0000294105"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         61
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305|Ref.2"
FT   BINDING         63
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000305|Ref.2"
FT   BINDING         104
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000305|Ref.2"
FT   BINDING         104
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="4"
FT                   /evidence="ECO:0000305|Ref.2"
FT   BINDING         153
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000305|Ref.2"
FT   BINDING         155
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="3"
FT                   /evidence="ECO:0000305|Ref.2"
FT   BINDING         207
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305|Ref.2"
FT   BINDING         209
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000305|Ref.2"
FT   BINDING         210
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000305|Ref.2"
FT   BINDING         258
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000305|Ref.2"
FT   BINDING         260
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000305|Ref.2"
SQ   SEQUENCE   286 AA;  32009 MW;  3CF1D97B9836057E CRC64;
     MTKSISLSKR IFVIVILFVI VAVCTFFVQS CARKSNHAAS FQNYHATIDG KEIAGITNNI
     SSLTWSAQSN TLFSTINKPA AIVEMTTNGD LIRTIPLDFV KDLETIEYIG DNQFVISDER
     DYAIYVISLT PNSEVKILKK IKIPLQESPT NCGFEGLAYS RQDHTFWFFK EKNPIEVYKV
     NGLLSSNELH ISKDKALQRQ FTLDDVSGAE FNQQKNTLLV LSHESRALQE VTLVGEVIGE
     MSLTKGSRGL SHNIKQAEGV AMDASGNIYI VSEPNRFYRF TPQSSH