CBIM_SALTY
ID CBIM_SALTY Reviewed; 245 AA.
AC Q05594;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Cobalt transport protein CbiM;
DE AltName: Full=Energy-coupling factor transporter probable substrate-capture protein CbiM;
DE Short=ECF transporter S component CbiM;
DE Flags: Precursor;
GN Name=cbiM; OrderedLocusNames=STM2023;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=8501034; DOI=10.1128/jb.175.11.3303-3316.1993;
RA Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M.;
RT "Characterization of the cobalamin (vitamin B12) biosynthetic genes of
RT Salmonella typhimurium.";
RL J. Bacteriol. 175:3303-3316(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP FUNCTION IN COBALT TRANSPORT, SUBSTRATES, SUBUNIT, AND EXPRESSION IN
RP E.COLI.
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=16352848; DOI=10.1128/jb.188.1.317-327.2006;
RA Rodionov D.A., Hebbeln P., Gelfand M.S., Eitinger T.;
RT "Comparative and functional genomic analysis of prokaryotic nickel and
RT cobalt uptake transporters: evidence for a novel group of ATP-binding
RT cassette transporters.";
RL J. Bacteriol. 188:317-327(2006).
CC -!- FUNCTION: Part of the energy-coupling factor (ECF) transporter complex
CC CbiMNOQ involved in cobalt import. The complex confers cobalt uptake
CC upon expression in E.coli; can also transport nickel with a very low
CC affinity. {ECO:0000269|PubMed:16352848}.
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC -!- SUBUNIT: Forms an energy-coupling factor (ECF) transporter complex
CC composed of an ATP-binding protein (A component, CbiO), a transmembrane
CC protein (T component, CbiQ) and 2 possible substrate-capture proteins
CC (S components, CbiM and CbiN) of unknown stoichimetry. Expression of
CC just CbiMN in E.coli confers some cobalt uptake.
CC {ECO:0000269|PubMed:16352848}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CbiM family. {ECO:0000305}.
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DR EMBL; L12006; AAA27264.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL20927.1; -; Genomic_DNA.
DR RefSeq; NP_460968.3; NC_003197.2.
DR RefSeq; WP_000764336.1; NC_003197.2.
DR AlphaFoldDB; Q05594; -.
DR SMR; Q05594; -.
DR STRING; 99287.STM2023; -.
DR TCDB; 3.A.1.23.6; the atp-binding cassette (abc) superfamily.
DR PaxDb; Q05594; -.
DR EnsemblBacteria; AAL20927; AAL20927; STM2023.
DR GeneID; 1253544; -.
DR KEGG; stm:STM2023; -.
DR HOGENOM; CLU_052508_3_0_6; -.
DR OMA; ANVFSMG; -.
DR PhylomeDB; Q05594; -.
DR BioCyc; SENT99287:STM2023-MON; -.
DR UniPathway; UPA00148; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IDA:UniProtKB.
DR GO; GO:0015087; F:cobalt ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006824; P:cobalt ion transport; IDA:UniProtKB.
DR HAMAP; MF_01462; CbiM; 1.
DR InterPro; IPR018024; CbiM.
DR InterPro; IPR002751; CbiM/NikMN.
DR PANTHER; PTHR43627; PTHR43627; 1.
DR Pfam; PF01891; CbiM; 1.
DR TIGRFAMs; TIGR00123; cbiM; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Cobalamin biosynthesis; Cobalt;
KW Cobalt transport; Ion transport; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..245
FT /note="Cobalt transport protein CbiM"
FT /id="PRO_0000089375"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 245 AA; 26299 MW; C1D1CC8D31E21F2C CRC64;
MKLEQQLRQL SFSGLAAALL LMVVPQQAFA MHIMEGFLPP VWALAWWLLF LPCLWYGLVR
LRRIVQEDNH QKVLLALCGA FIFVLSALKI PSVTGSCSHP TGVGLAVILF GPGVVAILGA
VVLLFQALLL AHGGLTTLGA NGMSMAVIGP VVGYLVWKMA CRAGLRRDVA VFLCAMLADL
ATYFVTSVQL GVAFPDPHAG ATGSVVKFMG IFCLTQIPVA IAEGLLTVMI YDQLTKRQVI
TVQGH
