ID   YK99_SCHPO              Reviewed;         647 AA.
AC   Q9HE06;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Putative pre-mRNA-splicing factor ATP-dependent RNA helicase C20H4.09;
DE            EC=3.6.4.13;
DE   AltName: Full=DEAH-box helicase C20H4.09;
GN   ORFNames=SPAC20H4.09;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Pre-mRNA processing factor involved in disassembly of
CC       spliceosomes after the release of mature mRNA.
CC       {ECO:0000250|UniProtKB:P53131}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC       {ECO:0000255}.
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DR   EMBL; CU329670; CAC19739.1; -; Genomic_DNA.
DR   RefSeq; NP_593629.1; NM_001019060.1.
DR   AlphaFoldDB; Q9HE06; -.
DR   SMR; Q9HE06; -.
DR   BioGRID; 279319; 22.
DR   STRING; 4896.SPAC20H4.09.1; -.
DR   MaxQB; Q9HE06; -.
DR   PaxDb; Q9HE06; -.
DR   PRIDE; Q9HE06; -.
DR   EnsemblFungi; SPAC20H4.09.1; SPAC20H4.09.1:pep; SPAC20H4.09.
DR   GeneID; 2542874; -.
DR   KEGG; spo:SPAC20H4.09; -.
DR   PomBase; SPAC20H4.09; -.
DR   VEuPathDB; FungiDB:SPAC20H4.09; -.
DR   eggNOG; KOG0922; Eukaryota.
DR   HOGENOM; CLU_001832_5_11_1; -.
DR   InParanoid; Q9HE06; -.
DR   OMA; STEQQMY; -.
DR   PhylomeDB; Q9HE06; -.
DR   PRO; PR:Q9HE06; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0005681; C:spliceosomal complex; ISS:PomBase.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; ISS:UniProtKB.
DR   GO; GO:0045292; P:mRNA cis splicing, via spliceosome; ISS:PomBase.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR   InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR   InterPro; IPR007502; Helicase-assoc_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF04408; HA2; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF07717; OB_NTP_bind; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00847; HA2; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; mRNA processing; mRNA splicing;
KW   Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN           1..647
FT                   /note="Putative pre-mRNA-splicing factor ATP-dependent RNA
FT                   helicase C20H4.09"
FT                   /id="PRO_0000055171"
FT   DOMAIN          35..199
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          219..398
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           146..149
FT                   /note="DEAH box"
FT   BINDING         48..55
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   647 AA;  73033 MW;  175C96A560E93969 CRC64;
     MPNEFISQSL STLTNTPLNI QKKLLPITKY RNQLLYAVEQ NQITIVLGHT GCGKTTQIPQ
     FLYEAGWASQ NGIIGCTQPR RLVAKSVSER VSLELNSPPG SLCGYSIQFD HNVSEKTKIK
     YMTDGILLNE IFFDPLLERY SIVILDEVHE RTLSTDLLLG VLKRILEKRN DFRLVLSSAS
     VDANKLSQFF GQDKVCTMSI EGKLFPVETL FLQKPTENYV DSAIETVINI NSTYPPGDIL
     VFLSGRKEIE YCIKKIEDSL IHASEDCQTL VPLPLHAGLT VDEQMRVFNI YDGDFRKVIF
     STNIAETSIT IDGIVYVVDS GFNKQRIYNP YTRTSKLINV PISKSSAIQR SGRAGRTMRG
     KVFRLYTEKA YSLMKEEFEA DILNCDMSPL VLFLKGLGLK NILQFPFFVR PPTVHLMAAL
     EDLYLLGVLD ESGNLTDPLG IQISNSFLDA NISKALLTSN QFGCTHEILS IASILTAGEV
     FYNPTSSSKN DAFVAHSSFF ANEGDIITAL NVFESFVGNK KDLQWCRKNY LNYQTLRQAL
     DIRTHLVRFL NKFSIPTAQR LPSSDCSKIL KCLLDGFVRN VAHLQNDGSY KTIGGKQVWL
     DSSSVLHEKK TPWIMYSSAV ESETQIFVKN ISKIESFWLD KYYKREK