YKE4_YEAST
ID YKE4_YEAST Reviewed; 346 AA.
AC P40544; D6VVQ6;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Zinc transporter YKE4;
GN Name=YKE4; OrderedLocusNames=YIL023C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16760462; DOI=10.1074/jbc.m604730200;
RA Kumanovics A., Poruk K.E., Osborn K.A., Ward D.M., Kaplan J.;
RT "YKE4 (YIL023C) encodes a bidirectional zinc transporter in the endoplasmic
RT reticulum of Saccharomyces cerevisiae.";
RL J. Biol. Chem. 281:22566-22574(2006).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Zinc transporter whose role depends on the zinc status of the
CC cells. It helps to balance zinc levels between the cytosol and the
CC secretory pathway. It transports zinc into the secretory pathway in a
CC zinc-adequate environment and in a high zinc medium. In high zinc
CC medium, transport of zinc into the secretory pathway is a way to
CC eliminate zinc from the cytosol. Under low cytosolic zinc conditions,
CC it removes zinc from the secretory pathway and acts as zinc importer
CC that helps to alleviate ER stress. {ECO:0000269|PubMed:16760462}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:16760462}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16760462}.
CC -!- SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family.
CC KE4/Catsup subfamily. {ECO:0000305}.
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DR EMBL; Z46881; CAA86969.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08522.1; -; Genomic_DNA.
DR PIR; S49959; S49959.
DR RefSeq; NP_012241.1; NM_001179373.1.
DR AlphaFoldDB; P40544; -.
DR SMR; P40544; -.
DR BioGRID; 34965; 56.
DR DIP; DIP-5638N; -.
DR STRING; 4932.YIL023C; -.
DR TCDB; 2.A.5.4.4; the zinc (zn(2+))-iron (fe(2+)) permease (zip) family.
DR PaxDb; P40544; -.
DR PRIDE; P40544; -.
DR EnsemblFungi; YIL023C_mRNA; YIL023C; YIL023C.
DR GeneID; 854789; -.
DR KEGG; sce:YIL023C; -.
DR SGD; S000001285; YKE4.
DR VEuPathDB; FungiDB:YIL023C; -.
DR eggNOG; KOG2693; Eukaryota.
DR GeneTree; ENSGT00940000157349; -.
DR HOGENOM; CLU_015114_0_0_1; -.
DR InParanoid; P40544; -.
DR OMA; IIQLMPC; -.
DR BioCyc; YEAST:G3O-31298-MON; -.
DR Reactome; R-SCE-442380; Zinc influx into cells by the SLC39 gene family.
DR PRO; PR:P40544; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P40544; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IMP:SGD.
DR GO; GO:0006882; P:cellular zinc ion homeostasis; IBA:GO_Central.
DR GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006829; P:zinc ion transport; IMP:SGD.
DR InterPro; IPR003689; ZIP.
DR Pfam; PF02535; Zip; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Glycoprotein; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport; Zinc;
KW Zinc transport.
FT CHAIN 1..346
FT /note="Zinc transporter YKE4"
FT /id="PRO_0000213698"
FT TOPO_DOM 1..2
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 24..69
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 91..99
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..126
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..202
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 224..252
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 274..290
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..322
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 344..346
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 184
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 274
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 285
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 346 AA; 37418 MW; 645960E194778006 CRC64;
MKASHICSYL LSIAPLVVSH GVHHNRDHGH EANHESKQSF LILKQESIFY SLVCFLQNHL
FVLGPRYNAI VAILIIQLMP CLFVLFVPGL RKNDRASLTL SLLVSFSLGT LLGDILLHVI
PESLSGVTDV TMVGGAIFLG FISFLTLDKT MRILSGTSND DGSIHSHSHS HTPQQTAEKK
AGFNMSAYLN VISGIAHHIT DGIALATSFY SSTQVGIMTS IAVTFHEIPH ELGDFAILLS
SGFTFPQAIR AQAVTAFGAV VGTSIGCWMN EIGNNSHKAT SSSANASELM LPFTAGGLIY
IATTSVVPQI LHSSAPDSKL REFKKWALQL VFIFVGFAVM ALMDEH
