ID   YKFA_ECOLI              Reviewed;         287 AA.
AC   P75678; P71287; Q47687;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   03-AUG-2022, entry version 142.
DE   RecName: Full=Uncharacterized protein YkfA;
GN   Name=ykfA; OrderedLocusNames=b0253, JW0243;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA   Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T.,
RA   Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S.,
RA   Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.;
RT   "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0
RT   - 6.0 min (189,987 - 281,416bp) region.";
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP   143-144 AND 165-287.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- SIMILARITY: To E.coli YfjP and YeeP. {ECO:0000305}.
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DR   EMBL; U70214; AAB08672.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73356.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAA77923.2; -; Genomic_DNA.
DR   PIR; E64750; E64750.
DR   RefSeq; NP_414787.4; NC_000913.3.
DR   RefSeq; WP_001065553.1; NZ_LN832404.1.
DR   AlphaFoldDB; P75678; -.
DR   BioGRID; 4262798; 13.
DR   IntAct; P75678; 1.
DR   STRING; 511145.b0253; -.
DR   PaxDb; P75678; -.
DR   PRIDE; P75678; -.
DR   EnsemblBacteria; AAC73356; AAC73356; b0253.
DR   EnsemblBacteria; BAA77923; BAA77923; BAA77923.
DR   GeneID; 63141112; -.
DR   GeneID; 949004; -.
DR   KEGG; ecj:JW0243; -.
DR   KEGG; eco:b0253; -.
DR   PATRIC; fig|511145.12.peg.256; -.
DR   EchoBASE; EB3123; -.
DR   eggNOG; COG3596; Bacteria.
DR   HOGENOM; CLU_059925_0_0_6; -.
DR   InParanoid; P75678; -.
DR   OMA; ARAVWDW; -.
DR   PhylomeDB; P75678; -.
DR   BioCyc; EcoCyc:G6128-MON; -.
DR   PRO; PR:P75678; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR006073; GTP-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01926; MMR_HSR1; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   4: Predicted;
KW   GTP-binding; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..287
FT                   /note="Uncharacterized protein YkfA"
FT                   /id="PRO_0000168547"
FT   DOMAIN          48..138
FT                   /note="G"
FT   BINDING         43..50
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
FT   BINDING         90..93
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
FT   BINDING         156..159
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   287 AA;  31892 MW;  38864691DD0B9C60 CRC64;
     MNNSEGLKSF QQSLADLPQW VSERLLQQIN QLTNYEPVIG IMGKTGVGKS SLCNALFAGD
     ISPVSDVAAC TREPLRFRLQ VGDRYITLMD LPGVGESGAR DTEYAALYRE QLPRLDLVLW
     LIKADDRALT VDEHFYHQVI GEVYRHKVLF VISQSDKAEP TSGGGQLSTA QKQNISRKIC
     LLHELFQPVH PVCAVSVRLQ WGLKVMAERM IKCLPREATS PVVSQLHPSF RTTVVREQAR
     SDFGETVGAV LDSISAFPLI PAPVRAVIQA VRTTVVSVAR AVWDFFF