YKFI_ECOLI
ID YKFI_ECOLI Reviewed; 113 AA.
AC P77692; Q9XB43;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Toxin YkfI {ECO:0000303|PubMed:14594833};
GN Name=ykfI; OrderedLocusNames=b0245, JW0234;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION AS A TOXIN.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=14594833; DOI=10.1128/jb.185.22.6600-6608.2003;
RA Brown J.M., Shaw K.J.;
RT "A novel family of Escherichia coli toxin-antitoxin gene pairs.";
RL J. Bacteriol. 185:6600-6608(2003).
RN [5]
RP FUNCTION AS A TOXIN, INTERACTION WITH FTSZ, AND MUTAGENESIS OF PHE-65.
RX PubMed=28931012; DOI=10.1371/journal.pgen.1007007;
RA Heller D.M., Tavag M., Hochschild A.;
RT "CbtA toxin of Escherichia coli inhibits cell division and cell elongation
RT via direct and independent interactions with FtsZ and MreB.";
RL PLoS Genet. 13:E1007007-E1007007(2017).
RN [6]
RP FUNCTION AS A TOXIN, INTERACTION WITH FTSZ, INDUCTION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=K12 / BW25113;
RX PubMed=28257056; DOI=10.3390/toxins9030077;
RA Wen Z., Wang P., Sun C., Guo Y., Wang X.;
RT "Interaction of type IV toxin/antitoxin systems in cryptic prophages of
RT Escherichia coli K-12.";
RL Toxins 9:0-0(2017).
CC -!- FUNCTION: Toxic component of a type IV toxin-antitoxin (TA) system
CC (PubMed:14594833, PubMed:28257056, PubMed:28931012). Acts as a toxin
CC inhibitor that blocks cell division and cell elongation via FtsZ and
CC possibly also MreB (although no interaction with MreB has been proven)
CC (PubMed:28931012). Overexpression results in inhibition of growth in
CC liquid cultures and a decrease in colony formation (PubMed:14594833,
CC PubMed:28257056, PubMed:28931012). These effects are overcome by
CC concomitant expression of cognate antitoxin YafW, which leads to toxin
CC loss by an unknown mechanism (PubMed:14594833, PubMed:28257056).
CC Overexpression leads to formation of lemon-shaped cells and cell lysis;
CC inactivated by overexpression of cognate antitoxin YafW but not when
CC the 2 genes are coexpressed from the same plasmid (PubMed:28257056).
CC Also neutralized by overexpression of non-cognate antitoxins YfjZ and
CC CbeA (PubMed:28257056). Co-overexpression of toxin YkfI and antitoxin
CC YafW leads to formation of elongated cells (PubMed:28257056).
CC {ECO:0000269|PubMed:14594833, ECO:0000269|PubMed:28257056,
CC ECO:0000269|PubMed:28931012}.
CC -!- SUBUNIT: Interacts with FtsZ but not MreB.
CC {ECO:0000269|PubMed:28257056, ECO:0000269|PubMed:28931012}.
CC -!- INDUCTION: Expressed in mid-log phase at lower levels than toxin relE.
CC {ECO:0000269|PubMed:28257056}.
CC -!- DISRUPTION PHENOTYPE: Deletion of 3 type IV toxin genes (cbtA, ykfI,
CC ypfJ) leads to a slight reduction in resistance to oxidative stress,
CC has no effect on cell growth. {ECO:0000269|PubMed:28257056}.
CC -!- MISCELLANEOUS: Encoded in prophage CP4-6. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CbtA/YkfI/YpjF toxin family. {ECO:0000305}.
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DR EMBL; U70214; AAB08664.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73348.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA77913.2; -; Genomic_DNA.
DR PIR; E64749; E64749.
DR RefSeq; NP_414779.1; NC_000913.3.
DR RefSeq; WP_000854672.1; NZ_LN832404.1.
DR AlphaFoldDB; P77692; -.
DR BioGRID; 4263161; 78.
DR BioGRID; 851067; 3.
DR IntAct; P77692; 3.
DR STRING; 511145.b0245; -.
DR PaxDb; P77692; -.
DR PRIDE; P77692; -.
DR EnsemblBacteria; AAC73348; AAC73348; b0245.
DR EnsemblBacteria; BAA77913; BAA77913; BAA77913.
DR GeneID; 946726; -.
DR KEGG; ecj:JW0234; -.
DR KEGG; eco:b0245; -.
DR PATRIC; fig|1411691.4.peg.2038; -.
DR EchoBASE; EB4079; -.
DR eggNOG; ENOG5030CTH; Bacteria.
DR HOGENOM; CLU_129204_1_1_6; -.
DR OMA; WQEQSPF; -.
DR PhylomeDB; P77692; -.
DR BioCyc; EcoCyc:G6120-MON; -.
DR PRO; PR:P77692; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0030308; P:negative regulation of cell growth; IMP:EcoCyc.
DR InterPro; IPR009610; CbtA_toxin.
DR Pfam; PF06755; CbtA_toxin; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Toxin-antitoxin system.
FT CHAIN 1..113
FT /note="Toxin YkfI"
FT /id="PRO_0000168551"
FT MUTAGEN 65
FT /note="F->S: Loss of interaction with FtsZ."
FT /evidence="ECO:0000269|PubMed:28931012"
SQ SEQUENCE 113 AA; 12905 MW; D0B15FBC0BF731B2 CRC64;
MKTLPAITQR AVKPCLSPVA VWQMLLTRLL EQHYGLTIND TPFCNEAVIK EHIDAGITLA
DAVNFLVEKY ELVRIDRKGF SWQEQSPYLR AADILRARQA TGLLRQSRNN VVR
