ID   YKGE_ECOLI              Reviewed;         239 AA.
AC   P77252; Q2MCB6;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Uncharacterized protein YkgE;
GN   Name=ykgE; OrderedLocusNames=b0306, JW5041;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   LACK OF COMPLEMENTATION OF B.SUBTILIS LUTABC OPERON.
RC   STRAIN=K12;
RX   PubMed=19201793; DOI=10.1128/jb.01464-08;
RA   Chai Y., Kolter R., Losick R.;
RT   "A widely conserved gene cluster required for lactate utilization in
RT   Bacillus subtilis and its involvement in biofilm formation.";
RL   J. Bacteriol. 191:2423-2430(2009).
CC   -!- MISCELLANEOUS: The E.coli ykgEFG operon is a clear homolog of the
CC       B.subtilis lutABC operon, however it is not able of restoring L-lactate
CC       utilization to the B.subtilis mutant for lutABC operon. Thus, ykgEFG
CC       operon may contribute to lactate catabolism in E.coli, but under
CC       conditions other than those tested, or alternatively, the ykgEFG operon
CC       may be responsible for the catabolism of a metabolite other than (but
CC       perhaps related to) lactate in E.coli.
CC   -!- SIMILARITY: Belongs to the LutA/YkgE family. {ECO:0000305}.
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DR   EMBL; U73857; AAB18033.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73409.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76090.1; -; Genomic_DNA.
DR   PIR; B64757; B64757.
DR   RefSeq; NP_414840.1; NC_000913.3.
DR   RefSeq; WP_001102108.1; NZ_SSZK01000067.1.
DR   AlphaFoldDB; P77252; -.
DR   SMR; P77252; -.
DR   BioGRID; 4262811; 20.
DR   STRING; 511145.b0306; -.
DR   jPOST; P77252; -.
DR   PaxDb; P77252; -.
DR   PRIDE; P77252; -.
DR   EnsemblBacteria; AAC73409; AAC73409; b0306.
DR   EnsemblBacteria; BAE76090; BAE76090; BAE76090.
DR   GeneID; 948438; -.
DR   KEGG; ecj:JW5041; -.
DR   KEGG; eco:b0306; -.
DR   PATRIC; fig|1411691.4.peg.1971; -.
DR   EchoBASE; EB3352; -.
DR   eggNOG; COG0247; Bacteria.
DR   HOGENOM; CLU_023081_1_0_6; -.
DR   InParanoid; P77252; -.
DR   OMA; NSCLMHI; -.
DR   PhylomeDB; P77252; -.
DR   BioCyc; EcoCyc:G6176-MON; -.
DR   PRO; PR:P77252; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IBA:GO_Central.
DR   InterPro; IPR004017; Cys_rich_dom.
DR   Pfam; PF02754; CCG; 2.
PE   3: Inferred from homology;
KW   Reference proteome.
FT   CHAIN           1..239
FT                   /note="Uncharacterized protein YkgE"
FT                   /id="PRO_0000168569"
SQ   SEQUENCE   239 AA;  26004 MW;  8CDCC8BBB8228B68 CRC64;
     MNVNFFVTCI GDALKSRMAR DSVLLLEKLG CRVNFPEKQG CCGQPAINSG YIKEAIPGMK
     NLIAALEDND DPIISPAGSC TYAVKSYPTY LADEPEWASR AAKVAARMQD LTSFIVNKLG
     VVDVGASLQG RAVYHPSCSL ARKLGVKDEP LTLLKNVRGL ELLTFAEQDT CCGFGGTFSV
     KMAEISGEMV KEKVAHLMEV RPEYLIGADV SCLLNISGRL QREGQKVKVM HIAEVLMSR