ID   CBIN_METTH              Reviewed;          95 AA.
AC   O26234;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 2.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Cobalt transport protein CbiN {ECO:0000255|HAMAP-Rule:MF_00330};
DE   AltName: Full=Energy-coupling factor transporter probable substrate-capture protein CbiN {ECO:0000255|HAMAP-Rule:MF_00330};
DE            Short=ECF transporter S component CbiN {ECO:0000255|HAMAP-Rule:MF_00330};
GN   Name=cbiN {ECO:0000255|HAMAP-Rule:MF_00330}; OrderedLocusNames=MTH_131;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: Part of the energy-coupling factor (ECF) transporter complex
CC       CbiMNOQ involved in cobalt import. {ECO:0000255|HAMAP-Rule:MF_00330}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00330}.
CC   -!- SUBUNIT: Forms an energy-coupling factor (ECF) transporter complex
CC       composed of an ATP-binding protein (A component, CbiO), a transmembrane
CC       protein (T component, CbiQ) and 2 possible substrate-capture proteins
CC       (S components, CbiM and CbiN) of unknown stoichimetry.
CC       {ECO:0000255|HAMAP-Rule:MF_00330}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00330};
CC       Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00330}.
CC   -!- SIMILARITY: Belongs to the CbiN family. {ECO:0000255|HAMAP-
CC       Rule:MF_00330}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB84637.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AE000666; AAB84637.1; ALT_FRAME; Genomic_DNA.
DR   PIR; A69041; A69041.
DR   AlphaFoldDB; O26234; -.
DR   STRING; 187420.MTH_131; -.
DR   EnsemblBacteria; AAB84637; AAB84637; MTH_131.
DR   KEGG; mth:MTH_131; -.
DR   PATRIC; fig|187420.15.peg.104; -.
DR   HOGENOM; CLU_136197_2_1_2; -.
DR   OMA; TEIEPDY; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015087; F:cobalt ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_00330; CbiN; 1.
DR   InterPro; IPR003705; CbiN.
DR   PANTHER; PTHR38662; PTHR38662; 1.
DR   Pfam; PF02553; CbiN; 1.
DR   TIGRFAMs; TIGR01165; cbiN; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cobalamin biosynthesis; Cobalt; Cobalt transport;
KW   Ion transport; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..95
FT                   /note="Cobalt transport protein CbiN"
FT                   /id="PRO_0000134703"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00330"
FT   TRANSMEM        67..87
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00330"
SQ   SEQUENCE   95 AA;  10310 MW;  589D8EB1686975AE CRC64;
     MDKRHILMLL AVIIISVAPL IIYSGHGEDD GYFGGADDSA GDAITETGYK PWFQPLWEPP
     SGEIESLLFA LQAAIGALII GYVFGYYRGR GESSE