CBIN_RHOCB
ID CBIN_RHOCB Reviewed; 103 AA.
AC O68104; D5AUZ8;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Cobalt transport protein CbiN;
DE AltName: Full=Energy-coupling factor transporter probable substrate-capture protein CbiN;
DE Short=ECF transporter S component CbiN;
GN Name=cbiN; OrderedLocusNames=RCAP_rcc02036;
OS Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Rhodobacter.
OX NCBI_TaxID=272942;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=9256491; DOI=10.1073/pnas.94.17.9384;
RA Vlcek C., Paces V., Maltsev N., Paces J., Haselkorn R., Fonstein M.;
RT "Sequence of a 189-kb segment of the chromosome of Rhodobacter capsulatus
RT SB1003.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:9384-9388(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=20418398; DOI=10.1128/jb.00366-10;
RA Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V.,
RA Haselkorn R.;
RT "Complete genome sequence of the photosynthetic purple nonsulfur bacterium
RT Rhodobacter capsulatus SB 1003.";
RL J. Bacteriol. 192:3545-3546(2010).
RN [3]
RP FUNCTION IN COBALT TRANSPORT, SUBSTRATES, SUBUNIT, AND EXPRESSION IN
RP E.COLI.
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=16352848; DOI=10.1128/jb.188.1.317-327.2006;
RA Rodionov D.A., Hebbeln P., Gelfand M.S., Eitinger T.;
RT "Comparative and functional genomic analysis of prokaryotic nickel and
RT cobalt uptake transporters: evidence for a novel group of ATP-binding
RT cassette transporters.";
RL J. Bacteriol. 188:317-327(2006).
RN [4]
RP FUNCTION IN COBALT TRANSPORT, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC BAA-309 / NBRC 16581 / SB1003;
RX PubMed=20868747; DOI=10.1016/j.resmic.2010.09.010;
RA Siche S., Neubauer O., Hebbeln P., Eitinger T.;
RT "A bipartite S unit of an ECF-type cobalt transporter.";
RL Res. Microbiol. 161:824-829(2010).
CC -!- FUNCTION: Part of the energy-coupling factor (ECF) transporter complex
CC CbiMNOQ involved in cobalt import. The complex confers cobalt uptake
CC upon expression in E.coli; can also transport nickel with a very low
CC affinity. A Cbi(MN) fusion protein has about 70% import capacity, but
CC the holo-Cbi(MN)QO complex cannot be isolated, suggesting CbiN may
CC destabilize it. {ECO:0000269|PubMed:16352848,
CC ECO:0000269|PubMed:20868747}.
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC -!- SUBUNIT: Forms an energy-coupling factor (ECF) transporter complex
CC composed of an ATP-binding protein (A component, CbiO), a transmembrane
CC protein (T component, CbiQ) and 2 possible substrate-capture proteins
CC (S components, CbiM and CbiN) of unknown stoichimetry. Subcomplexes
CC composed of CbiMQO can be isolated from membranes but the CbiN subunit
CC is not isolated in association with them, suggesting it is only loosely
CC associated. Expression of just CbiMN in E.coli confers some cobalt
CC uptake. {ECO:0000269|PubMed:16352848, ECO:0000269|PubMed:20868747}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CbiN family. {ECO:0000305}.
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DR EMBL; AF010496; AAC16194.1; -; Genomic_DNA.
DR EMBL; CP001312; ADE85780.1; -; Genomic_DNA.
DR PIR; T03541; T03541.
DR RefSeq; WP_013067759.1; NC_014034.1.
DR AlphaFoldDB; O68104; -.
DR STRING; 272942.RCAP_rcc02036; -.
DR TCDB; 3.A.1.23.8; the atp-binding cassette (abc) superfamily.
DR EnsemblBacteria; ADE85780; ADE85780; RCAP_rcc02036.
DR GeneID; 31490898; -.
DR KEGG; rcp:RCAP_rcc02036; -.
DR eggNOG; COG1930; Bacteria.
DR HOGENOM; CLU_136197_2_0_5; -.
DR OMA; TEIEPDY; -.
DR OrthoDB; 2073796at2; -.
DR UniPathway; UPA00148; -.
DR Proteomes; UP000002361; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IDA:UniProtKB.
DR GO; GO:0015087; F:cobalt ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006824; P:cobalt ion transport; IDA:UniProtKB.
DR HAMAP; MF_00330; CbiN; 1.
DR InterPro; IPR003705; CbiN.
DR PANTHER; PTHR38662; PTHR38662; 1.
DR Pfam; PF02553; CbiN; 1.
DR TIGRFAMs; TIGR01165; cbiN; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Cobalamin biosynthesis; Cobalt;
KW Cobalt transport; Ion transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..103
FT /note="Cobalt transport protein CbiN"
FT /id="PRO_0000134696"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 103 AA; 10732 MW; 6671324927DFCAE2 CRC64;
MSSKRTLWLL AGTVALVVVP LLMGGEFGGA DGQAAELIEA TVPGFAPWAD PLWEPPSGEV
ESLFFALQAA LGAFVVGLVI GRRQGAAKTR EQNAPAPRSF PAE
