ID   YKOJ_BACSU              Reviewed;         170 AA.
AC   O35012; Q796L5;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Uncharacterized protein YkoJ;
DE   Flags: Precursor;
GN   Name=ykoJ; OrderedLocusNames=BSU13280;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Devine K.M.;
RT   "Sequence of the Bacillus subtilis genome between xlyA and ykoR.";
RL   Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   INDUCTION, DISRUPTION PHENOTYPE, AND OVEREXPRESSION.
RC   STRAIN=168;
RX   PubMed=17209068; DOI=10.1128/aem.02356-06;
RA   Nijland R., Heerlien R., Hamoen L.W., Kuipers O.P.;
RT   "Changing a single amino acid in Clostridium perfringens beta-toxin affects
RT   the efficiency of heterologous secretion by Bacillus subtilis.";
RL   Appl. Environ. Microbiol. 73:1586-1593(2007).
RN   [4]
RP   DEVELOPMENTAL STAGE, AND INDUCTION.
RC   STRAIN=168;
RX   PubMed=17586671; DOI=10.1128/aem.00472-07;
RA   Lulko A.T., Veening J.-W., Buist G., Smits W.K., Blom E.J., Beekman A.C.,
RA   Bron S., Kuipers O.P.;
RT   "Production and secretion stress caused by overexpression of heterologous
RT   alpha-amylase leads to inhibition of sporulation and a prolonged motile
RT   phase in Bacillus subtilis.";
RL   Appl. Environ. Microbiol. 73:5354-5362(2007).
CC   -!- DEVELOPMENTAL STAGE: Highly expressed in the late exponential phase.
CC       {ECO:0000269|PubMed:17586671}.
CC   -!- INDUCTION: Up-regulated under secretion stress conditions in a
CC       CssR/CssS-dependent manner. {ECO:0000269|PubMed:17209068,
CC       ECO:0000269|PubMed:17586671}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene show no noteworthy
CC       improvement of beta-toxoid secretion. {ECO:0000269|PubMed:17209068}.
CC   -!- MISCELLANEOUS: Overexpression of ykoJ is lethal to B.subtilis.
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DR   EMBL; AJ002571; CAA05607.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB13185.1; -; Genomic_DNA.
DR   PIR; F69859; F69859.
DR   RefSeq; NP_389211.1; NC_000964.3.
DR   RefSeq; WP_003232544.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; O35012; -.
DR   SMR; O35012; -.
DR   STRING; 224308.BSU13280; -.
DR   PaxDb; O35012; -.
DR   PRIDE; O35012; -.
DR   DNASU; 936459; -.
DR   EnsemblBacteria; CAB13185; CAB13185; BSU_13280.
DR   GeneID; 936459; -.
DR   KEGG; bsu:BSU13280; -.
DR   PATRIC; fig|224308.179.peg.1442; -.
DR   eggNOG; COG3212; Bacteria.
DR   InParanoid; O35012; -.
DR   OMA; YNAFAKE; -.
DR   BioCyc; BSUB:BSU13280-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   InterPro; IPR025711; PepSY.
DR   Pfam; PF03413; PepSY; 2.
PE   2: Evidence at transcript level;
KW   Reference proteome; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..170
FT                   /note="Uncharacterized protein YkoJ"
FT                   /id="PRO_0000383596"
SQ   SEQUENCE   170 AA;  18955 MW;  8C71F46E332EFE74 CRC64;
     MLKKKWMVGL LAGCLAAGGF SYNAFATENN ENRQASSKTD ALTEQEAEAI AKTVVDGTVE
     DIDRDLYNGK EVYEVEIEKE GEDYDVYVDI HTKQALNDPL KEKAEQVAIT KEEAEEIALK
     QTGGTVTESK LDEDDGAYIY EMEIQTKQGT ETEFEISAKD GRIIKQEIDD