YKOW_BACSU
ID YKOW_BACSU Reviewed; 800 AA.
AC O34311;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Signaling protein YkoW;
GN Name=ykoW; OrderedLocusNames=BSU13420;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [2]
RP IDENTIFICATION OF INITIATOR METHIONINE, DISCUSSION OF SEQUENCE, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=11728710; DOI=10.1111/j.1574-6968.2001.tb10919.x;
RA Galperin M.Y., Gaidenko T.A., Mulkidjanian A.Y., Nakano M., Price C.W.;
RT "MHYT, a new integral membrane sensor domain.";
RL FEMS Microbiol. Lett. 205:17-23(2001).
CC -!- FUNCTION: Probable signaling protein whose physiological role is not
CC yet known.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255|PROSITE-ProRule:PRU00244}.
CC -!- DISRUPTION PHENOTYPE: Cells do not display any phenotype.
CC {ECO:0000269|PubMed:11728710}.
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DR EMBL; AL009126; CAB13199.2; -; Genomic_DNA.
DR PIR; A69861; A69861.
DR RefSeq; WP_009967094.1; NZ_JNCM01000035.1.
DR RefSeq; YP_054577.1; NC_000964.3.
DR AlphaFoldDB; O34311; -.
DR SMR; O34311; -.
DR STRING; 224308.BSU13420; -.
DR PaxDb; O34311; -.
DR PRIDE; O34311; -.
DR EnsemblBacteria; CAB13199; CAB13199; BSU_13420.
DR GeneID; 2914252; -.
DR KEGG; bsu:BSU13420; -.
DR PATRIC; fig|224308.179.peg.1457; -.
DR eggNOG; COG3300; Bacteria.
DR eggNOG; COG5001; Bacteria.
DR InParanoid; O34311; -.
DR OMA; RMVMTTS; -.
DR BioCyc; BSUB:BSU13420-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR CDD; cd01948; EAL; 1.
DR CDD; cd01949; GGDEF; 1.
DR Gene3D; 3.20.20.450; -; 1.
DR Gene3D; 3.30.70.270; -; 1.
DR InterPro; IPR001633; EAL_dom.
DR InterPro; IPR035919; EAL_sf.
DR InterPro; IPR000160; GGDEF_dom.
DR InterPro; IPR005330; MHYT_dom.
DR InterPro; IPR029787; Nucleotide_cyclase.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR Pfam; PF00563; EAL; 1.
DR Pfam; PF00990; GGDEF; 1.
DR Pfam; PF03707; MHYT; 3.
DR SMART; SM00052; EAL; 1.
DR SMART; SM00267; GGDEF; 1.
DR SUPFAM; SSF141868; SSF141868; 1.
DR SUPFAM; SSF55073; SSF55073; 1.
DR SUPFAM; SSF55785; SSF55785; 1.
DR TIGRFAMs; TIGR00254; GGDEF; 1.
DR PROSITE; PS50883; EAL; 1.
DR PROSITE; PS50887; GGDEF; 1.
DR PROSITE; PS50924; MHYT; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..800
FT /note="Signaling protein YkoW"
FT /id="PRO_0000170472"
FT TRANSMEM 5..27
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00244"
FT TRANSMEM 44..66
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00244"
FT TRANSMEM 76..98
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00244"
FT TRANSMEM 103..125
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00244"
FT TRANSMEM 135..157
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00244"
FT TRANSMEM 178..200
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00244"
FT TRANSMEM 215..237
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00244"
FT DOMAIN 7..201
FT /note="MHYT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00244"
FT DOMAIN 255..319
FT /note="PAS"
FT DOMAIN 402..536
FT /note="GGDEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00095"
FT DOMAIN 545..798
FT /note="EAL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00074"
SQ SEQUENCE 800 AA; 90395 MW; 2613D29023FE903A CRC64;
MEIHVTYNTT LICLSILIAC TASYISLELS RKVTINTGLK SKIWLIGGSL IMGFGIWSMH
FVGMMAVHME MPMEYEFMPL MAAIGASVSG SFVSLYFVSR HILTYYRLLT GSVVLGASIA
SMHYIGMSAI SRVMIIYEPI LFTVSIIIAI AASFVSLKIF FDLAVKKHSE HLIFYKGVSS
IVMGIGISGM HYTGMLAATF HKDMAPPGSH MEVQTFHWSI FVTLIIFCIQ TLLLFSSHAD
RKFIKQSERI KDNEQRFQSL IVHNIDAIFI LSLEGDIISS NHAGEEMISK FGFSMHDWRN
YTSLKVKRLF EQVKKDKQAM NSDSDLITEK GQFHLNITLI PVEVNQELDS IYVICKDMTK
QYKAEKEIHR MAHYDSLTDL PNRRHAISHL TKVLNREHSL HYNTVVFFLD LNRFKVINDA
LGHNVGDQLL QFAAKRLSSV VPDNGFIARL GGDEFIIILT DANTGTGEAD VLARKIIQKF
KKPFKIQDHT LVTSVSIGIA ISPKDGTDGL ELMKKADMAM YAAKERNKSK YRYYSFSIGN
KETVKLNQEM VLREAIENDR FVLHYQPQFS VKKQKMTGAE ALIRLVTPDG QLRPPGEFIG
VAEETGLIID IGKWIIDEAC KQARIWHDKG YDLSVAINIS ARQFQSKDLI PLIKDTLNKY
QLPPQLLEVE VTESMTMDNL NHSKKVLSSL TELGIRISID DFGTGHSSLS YLKDFPIHRL
KIDKSFIDDI QTHPKSEQIT GAIIAMGHQL SLQVIAEGVE NAAQKQLLFE KGCDHLQGFF
FSRPIPPEQF EQFIIEQPSQ
