ID   YKP4_KLULA              Reviewed;         597 AA.
AC   P05470;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Uncharacterized killer plasmid pGKL-2 helicase;
DE            EC=3.6.4.-;
DE   AltName: Full=Plasmid pGKL-2 protein 4;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OG   Plasmid pGKl-2.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=3041369; DOI=10.1093/nar/16.13.5863;
RA   Tommasino M., Ricci S., Galeotti C.L.;
RT   "Genome organization of the killer plasmid pGKL2 from Kluyveromyces
RT   lactis.";
RL   Nucleic Acids Res. 16:5863-5878(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=3138657;
RA   Wilson D.W., Meacock P.A.;
RT   "Extranuclear gene expression in yeast: evidence for a plasmid-encoded RNA
RT   polymerase of unique structure.";
RL   Nucleic Acids Res. 16:8097-8112(1988).
CC   -!- FUNCTION: The presence of the two linear plasmids, termed pGKL1 and
CC       pGKL2, in strains of Kluyveromyces lactis confers the killer phenotype
CC       to the host cell, by promoting the secretion of a toxin able to inhibit
CC       the growth of sensitive strains.
CC   -!- SIMILARITY: Belongs to the helicase family. {ECO:0000305}.
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DR   EMBL; X07776; CAA30605.1; -; Genomic_DNA.
DR   EMBL; X07946; CAA30772.1; -; Genomic_DNA.
DR   PIR; S00962; S00962.
DR   AlphaFoldDB; P05470; -.
DR   Proteomes; UP000000598; Plasmid pGKL2.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Plasmid;
KW   Reference proteome.
FT   CHAIN           1..597
FT                   /note="Uncharacterized killer plasmid pGKL-2 helicase"
FT                   /id="PRO_0000102191"
FT   DOMAIN          48..198
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          275..467
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           146..149
FT                   /note="DEVH box"
FT   BINDING         61..68
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   CONFLICT        100
FT                   /note="N -> I (in Ref. 2; CAA30772)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        575
FT                   /note="I -> N (in Ref. 2; CAA30772)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   597 AA;  69165 MW;  83A6F61583DB8F11 CRC64;
     MVLKRVRCYY LIITIRLKMK VFTNKNLSIE QYGFINTFPN QNYILSYLHP YNPYSSLIVC
     YDVGLGKTYA AACLAHMYLD SGFKVLYLSN SLNSIDNFSN EYEKVVLDSR LNSLKKNITI
     KSFSKFYNCE KRGESDNVDY GLIILDEVHN LRESAYRYKL IKNKLDTMNN SKILVITATP
     MIDSKDELDS ILSLTKETSR IIFSENKIDI KISYVGQEIN GETLFLSEMK GQQLKEYLKV
     VNEENDTVYS SSRQASISLS NKFNPSIPLD EQSSKINAFI NSIKEGELTV LFSFYVKRGI
     DFTSSVLESI GYKKWNSNKK QKRTYAVIDG RTNKKNVEEI LTSFNSIANI KGDNIHILLG
     SSVLSESITL YRVKHLHIIS PFWNYGQIKQ SIGRAIRIGS HEGLEDKSMK VYLHAAHYDK
     EGKDIDIWKI AYDKNKDIIK RLEELKIDNE FSNDSLLDIP KIDNEMVIKI NEWVWDFRNC
     FDTNKFKISW CKIYEDKAIG YNLENNTKIM GNIPSYIRIN RPIPGGYTIW RSCIDKKLRI
     SFIDGEVNKF SKRGKLISNV NTSEIAKDLN CENNIESIIN TLKEQNRYFD KQIEYDL