ID   YKP9_KLULA              Reviewed;         453 AA.
AC   P05475;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Uncharacterized killer plasmid pGKl-2 protein 9;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OG   Plasmid pGKl-2.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=3041369; DOI=10.1093/nar/16.13.5863;
RA   Tommasino M., Ricci S., Galeotti C.L.;
RT   "Genome organization of the killer plasmid pGKL2 from Kluyveromyces
RT   lactis.";
RL   Nucleic Acids Res. 16:5863-5878(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=3138657;
RA   Wilson D.W., Meacock P.A.;
RT   "Extranuclear gene expression in yeast: evidence for a plasmid-encoded RNA
RT   polymerase of unique structure.";
RL   Nucleic Acids Res. 16:8097-8112(1988).
CC   -!- FUNCTION: The presence of the two linear plasmids, termed pGKL1 and
CC       pGKL2, in strains of Kluyveromyces lactis confers the killer phenotype
CC       to the host cell, by promoting the secretion of a toxin able to inhibit
CC       the growth of sensitive strains.
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DR   EMBL; X07776; CAA30609.1; -; Genomic_DNA.
DR   EMBL; X07946; CAA30768.1; -; Genomic_DNA.
DR   PIR; S00967; S00967.
DR   PIR; S10337; S10337.
DR   AlphaFoldDB; P05475; -.
DR   Proteomes; UP000000598; Plasmid pGKL2.
DR   InterPro; IPR035143; DUF5483.
DR   Pfam; PF17581; DUF5483; 1.
PE   4: Predicted;
KW   Plasmid; Reference proteome.
FT   CHAIN           1..453
FT                   /note="Uncharacterized killer plasmid pGKl-2 protein 9"
FT                   /id="PRO_0000066282"
FT   CONFLICT        289..290
FT                   /note="FI -> LK (in Ref. 2; CAA30768)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        296
FT                   /note="N -> F (in Ref. 2; CAA30768)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   453 AA;  55042 MW;  FC857D55B3EBD683 CRC64;
     MEIIEHKVEF KYKSIDEYQW TDNFKLDKQW EILRFKKNKI VQKSYMNRFP YSEPKKDYSI
     ALYSKNKWYC LKDGVGSYIS IQKEKPQIKD LTITEIKYEP YLVIYKLDFE YHIMMTILHC
     DERVKLLEYK RPFLKEGIIL FNEMKIYVYN GLFSYKGNKI EDFELFVNEF NSQYKENYDR
     LFKIFGKSNH THKSIDYKTW CKAKRENQQE WPLFTNIKIK DSIEFPNNSG IYYYYPDKYV
     GLARRNDDNL IPLIPKIYRK NHYENKNSLL YKYVNGLPID EEENIPYSFI KEKYKNIKYH
     EDEKIIEVDY NNNVVKQKDM AEYYCYNNKL IQKIEEPVKL PKIEAQILNK RNERVEVLID
     NEWKNLAGNR IDGIPTLPWN YKHIIHDYNK LGRLKKGKIM DLTHIGIVNE NGTDIITWPY
     TDDLYIGRSI ETKRLITFKY QKNVEIYDKL IDM