YKR18_YEAST
ID YKR18_YEAST Reviewed; 725 AA.
AC P36114; D6VX83;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=IML2-like protein YKR018C;
GN OrderedLocusNames=YKR018C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-380, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-196; SER-246 AND SER-380, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377 AND SER-380, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 54-57.
RX PubMed=12920126; DOI=10.1074/jbc.m304895200;
RA Schweizer A., Briand C., Grutter M.G.;
RT "Crystal structure of caspase-2, apical initiator of the intrinsic
RT apoptotic pathway.";
RL J. Biol. Chem. 278:42441-42447(2003).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 54-57.
RX PubMed=18780184; DOI=10.1007/s10495-008-0259-9;
RA Fu G., Chumanevich A.A., Agniswamy J., Fang B., Harrison R.W., Weber I.T.;
RT "Structural basis for executioner caspase recognition of P5 position in
RT substrates.";
RL Apoptosis 13:1291-1302(2008).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1770 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the IML2 family. {ECO:0000305}.
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DR EMBL; Z28243; CAA82090.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09173.1; -; Genomic_DNA.
DR PIR; S38087; S38087.
DR RefSeq; NP_012943.1; NM_001179808.1.
DR PDB; 1PYO; X-ray; 1.65 A; E/F=54-57.
DR PDB; 3EDR; X-ray; 2.45 A; E/F=54-57.
DR PDBsum; 1PYO; -.
DR PDBsum; 3EDR; -.
DR AlphaFoldDB; P36114; -.
DR SMR; P36114; -.
DR BioGRID; 34150; 140.
DR DIP; DIP-6636N; -.
DR IntAct; P36114; 2.
DR STRING; 4932.YKR018C; -.
DR iPTMnet; P36114; -.
DR MaxQB; P36114; -.
DR PaxDb; P36114; -.
DR PRIDE; P36114; -.
DR EnsemblFungi; YKR018C_mRNA; YKR018C; YKR018C.
DR GeneID; 853888; -.
DR KEGG; sce:YKR018C; -.
DR SGD; S000001726; YKR018C.
DR VEuPathDB; FungiDB:YKR018C; -.
DR eggNOG; KOG3783; Eukaryota.
DR GeneTree; ENSGT00940000176789; -.
DR HOGENOM; CLU_014926_0_0_1; -.
DR InParanoid; P36114; -.
DR OMA; FWNGHSR; -.
DR BioCyc; YEAST:G3O-31994-MON; -.
DR EvolutionaryTrace; P36114; -.
DR PRO; PR:P36114; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36114; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR InterPro; IPR019412; Iml2/TPR_39.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR31859; PTHR31859; 1.
DR Pfam; PF10300; DUF3808; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..725
FT /note="IML2-like protein YKR018C"
FT /id="PRO_0000203198"
FT MOD_RES 196
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 246
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 377
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 380
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"
SQ SEQUENCE 725 AA; 81754 MW; B2B91BAF52337043 CRC64;
MFKVFGFGAK EEIPELSQEE KTKAILKQAH DFEQALRAMD YVLDDNADEG LALLDESDAK
EASDQTINAL ARGVIEFLEA TLGFEAEEMK KASATLAKAE ALSLKSRERA QKIGLKSSSL
YPPGTVYAVT YTESCLLHAL LMIFSESMME AAKAILKLRK SYYMLQEILE TIKAANKAKK
LKITSGSEDK ESTPATFITG GDAFNSVDIP YELTPEEQKD KDLLQFAEQI HSMRTERLSG
AHIGNSPAIN RLRGELGLQA MEDLPEEEIT DHKVLSDDID LSQATIDEFV HSGVNLCFGI
LQVVISLLPP AIGAVLSVVG FRGSREEGLR LVWKATKQRN VHGCIGLLAL MFYYDGPFQF
TDDDFDIPAA VKDSSNSEDS EDEEMDGPTL LHPGKILEDA LLQSRALFPN SALWLLNEAR
MLSGKGRLEE AVALMDSIDV SKIRMRQVKS LMIFDRAITL IHLHQYDRAA EDILSLLDIS
DWSHAFYTYF AGCCYLENWR MCEMGLMKSD KKDEYQKKAE ELIFTSVNLL GKKTFKSKNL
PLDRFILRKV EQFKAKKEEL GVENPLDGIA TSPVHEIAYF YNGYNRMSEE HLELTKKMLT
EYRNPAIEAL DSDQELIKDL LVSLTLRRLG HIQEGCDILD EKVLPKFFSI QNGKVKYIKK
TEDPWAYPTA LYERALFTWK LEGMDGLPES KEWLLRAQGY ADDYELSTRV GMKIKAAIDR
VDHSL
