ID   YKUT_BACSU              Reviewed;         267 AA.
AC   O34897; Q796J9;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Uncharacterized MscS family protein YkuT;
GN   Name=ykuT; OrderedLocusNames=BSU14210;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Scanlan E., Devine K.M.;
RT   "Sequence of the Bacillus subtilis chromosome from ykuA to cse-15.";
RL   Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 157.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168 / JH642;
RX   PubMed=18310427; DOI=10.1128/aem.01573-07;
RA   Hoffmann T., Boiangiu C., Moses S., Bremer E.;
RT   "Responses of Bacillus subtilis to hypotonic challenges: physiological
RT   contributions of mechanosensitive channels to cellular survival.";
RL   Appl. Environ. Microbiol. 74:2454-2460(2008).
RN   [5]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168 / PS832;
RX   PubMed=17665170; DOI=10.1007/s00203-007-0292-z;
RA   Wahome P.G., Setlow P.;
RT   "Growth, osmotic downshock resistance and differentiation of Bacillus
RT   subtilis strains lacking mechanosensitive channels.";
RL   Arch. Microbiol. 189:49-58(2008).
CC   -!- FUNCTION: May play a role in resistance to osmotic downshock.
CC       {ECO:0000269|PubMed:17665170}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Expression is sigma B-dependent. Induced by high salt
CC       concentrations. {ECO:0000269|PubMed:17665170,
CC       ECO:0000269|PubMed:18310427}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene grow normally in minimal
CC       medium or in high-osmolarity environments, and exhibit the same
CC       survival capacity as the wild-type strain upon a drastic osmotic
CC       downshift. They sporulate and germinate normally. Combination with an
CC       MscL mutation enhances the osmosensitivity of MscL mutants subsequent
CC       to a hypo-osmotic shock. {ECO:0000269|PubMed:17665170,
CC       ECO:0000269|PubMed:18310427}.
CC   -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
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DR   EMBL; AJ222587; CAA10883.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB13294.2; -; Genomic_DNA.
DR   PIR; A69867; A69867.
DR   RefSeq; NP_389304.2; NC_000964.3.
DR   RefSeq; WP_003244994.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; O34897; -.
DR   SMR; O34897; -.
DR   STRING; 224308.BSU14210; -.
DR   TCDB; 1.A.23.3.1; the small conductance mechanosensitive ion channel (mscs) family.
DR   PaxDb; O34897; -.
DR   PRIDE; O34897; -.
DR   EnsemblBacteria; CAB13294; CAB13294; BSU_14210.
DR   GeneID; 938809; -.
DR   KEGG; bsu:BSU14210; -.
DR   PATRIC; fig|224308.179.peg.1550; -.
DR   eggNOG; COG0668; Bacteria.
DR   InParanoid; O34897; -.
DR   OMA; EASKHRQ; -.
DR   PhylomeDB; O34897; -.
DR   BioCyc; BSUB:BSU14210-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR   GO; GO:0071470; P:cellular response to osmotic stress; IBA:GO_Central.
DR   Gene3D; 2.30.30.60; -; 1.
DR   InterPro; IPR010920; LSM_dom_sf.
DR   InterPro; IPR006685; MscS_channel.
DR   InterPro; IPR011066; MscS_channel_C.
DR   InterPro; IPR011014; MscS_channel_TM-2.
DR   InterPro; IPR023408; MscS_dom_sf.
DR   InterPro; IPR045276; YbiO_bact.
DR   PANTHER; PTHR30460; PTHR30460; 1.
DR   Pfam; PF00924; MS_channel; 1.
DR   SUPFAM; SSF50182; SSF50182; 1.
DR   SUPFAM; SSF82689; SSF82689; 1.
DR   SUPFAM; SSF82861; SSF82861; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..267
FT                   /note="Uncharacterized MscS family protein YkuT"
FT                   /id="PRO_0000367893"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        101..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        157
FT                   /note="G -> E (in Ref. 1; CAA10883)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   267 AA;  29956 MW;  112989D430F5A4B7 CRC64;
     MDFIKQYDWA GLITNAGVLL IKLAIMILLY FIVRSLGMKI IKHLFAKFEE QNSLSIGRAH
     TLRSLTLNIF AYTLIFIFFV MVLDLFHYDP SALLAGAGIV GLAVGFGAQG LVSDIVTGFF
     ILLEKQLDVG DYITVSTFDG IVEQVGLRTT QIRSFDGTLH YIPNRNITNV SNHSRGTMQA
     LVDIKVPAER NIDEMIHILQ QVCDETAAAL PQIKEGPNVI GIQELGTSEI VIRVIAKTEN
     MEQWRVERVL RKEIKNAFDR AFPKETE