ID   YKWC_BACSU              Reviewed;         288 AA.
AC   O34948;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 133.
DE   RecName: Full=Uncharacterized oxidoreductase YkwC;
DE            EC=1.1.-.-;
GN   Name=ykwC; OrderedLocusNames=BSU13960;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Scanlan E., Devine K.M.;
RT   "Sequence of the Bacillus subtilis chromosome from ykuA to cse-15.";
RL   Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / NRRL NRS-744 / VKM B-501;
RX   PubMed=22882210; DOI=10.1111/j.1365-2958.2012.08205.x;
RA   Yepes A., Schneider J., Mielich B., Koch G., Garcia-Betancur J.C.,
RA   Ramamurthi K.S., Vlamakis H., Lopez D.;
RT   "The biofilm formation defect of a Bacillus subtilis flotillin-defective
RT   mutant involves the protease FtsH.";
RL   Mol. Microbiol. 86:457-471(2012).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22882210}.
CC       Membrane raft {ECO:0000269|PubMed:22882210}. Note=Present in detergent-
CC       resistant membrane (DRM) fractions that may be equivalent to eukaryotic
CC       membrane rafts; these rafts include proteins involved in signaling,
CC       molecule trafficking and protein secretion.
CC       {ECO:0000269|PubMed:22882210}.
CC   -!- SIMILARITY: Belongs to the HIBADH-related family. {ECO:0000305}.
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DR   EMBL; AJ222587; CAA10859.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB13269.1; -; Genomic_DNA.
DR   PIR; B69870; B69870.
DR   RefSeq; NP_389279.1; NC_000964.3.
DR   RefSeq; WP_003245029.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; O34948; -.
DR   SMR; O34948; -.
DR   IntAct; O34948; 1.
DR   MINT; O34948; -.
DR   STRING; 224308.BSU13960; -.
DR   jPOST; O34948; -.
DR   PaxDb; O34948; -.
DR   PRIDE; O34948; -.
DR   EnsemblBacteria; CAB13269; CAB13269; BSU_13960.
DR   GeneID; 939723; -.
DR   KEGG; bsu:BSU13960; -.
DR   PATRIC; fig|224308.179.peg.1522; -.
DR   eggNOG; COG2084; Bacteria.
DR   InParanoid; O34948; -.
DR   OMA; WSSEVNN; -.
DR   PhylomeDB; O34948; -.
DR   BioCyc; BSUB:BSU13960-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016054; P:organic acid catabolic process; IEA:UniProt.
DR   Gene3D; 1.10.1040.10; -; 1.
DR   InterPro; IPR002204; 3-OH-isobutyrate_DH-rel_CS.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR006115; 6PGDH_NADP-bd.
DR   InterPro; IPR015815; HIBADH-related.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR029154; NADP-bd.
DR   Pfam; PF14833; NAD_binding_11; 1.
DR   Pfam; PF03446; NAD_binding_2; 1.
DR   PIRSF; PIRSF000103; HIBADH; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00895; 3_HYDROXYISOBUT_DH; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..288
FT                   /note="Uncharacterized oxidoreductase YkwC"
FT                   /id="PRO_0000173068"
FT   ACT_SITE        172
FT                   /evidence="ECO:0000250"
FT   BINDING         6..20
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         97
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         240
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   288 AA;  30711 MW;  976DD9098DB47A30 CRC64;
     MKKTIGFIGL GVMGKSMASH ILNDGHPVLV YTRTKEKAES ILQKGAIWKD TVKDLSKEAD
     VIITMVGYPS DVEEVYFGSN GIIENAKEGA YLIDMTTSKP SLAKKIAEAA KEKALFALDA
     PVSGGDIGAQ NGTLAIMVGG EKEAFEACMP IFSLMGENIQ YQGPAGSGQH TKMCNQIAIA
     AGMIGVAEAM AYAQKSGLEP ENVLKSITTG AAGSWSLSNL APRMLQGNFE PGFYVKHFIK
     DMGIALEEAE LMGEEMPGLS LAKSLYDKLA AQGEENSGTQ SIYKLWVK