CBIN_SALTY
ID CBIN_SALTY Reviewed; 93 AA.
AC Q05595;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Cobalt transport protein CbiN;
DE AltName: Full=Energy-coupling factor transporter probable substrate-capture protein CbiN;
DE Short=ECF transporter S component CbiN;
GN Name=cbiN; OrderedLocusNames=STM2022;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=8501034; DOI=10.1128/jb.175.11.3303-3316.1993;
RA Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M.;
RT "Characterization of the cobalamin (vitamin B12) biosynthetic genes of
RT Salmonella typhimurium.";
RL J. Bacteriol. 175:3303-3316(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP FUNCTION IN COBALT TRANSPORT, SUBSTRATES, SUBUNIT, AND EXPRESSION IN
RP E.COLI.
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=16352848; DOI=10.1128/jb.188.1.317-327.2006;
RA Rodionov D.A., Hebbeln P., Gelfand M.S., Eitinger T.;
RT "Comparative and functional genomic analysis of prokaryotic nickel and
RT cobalt uptake transporters: evidence for a novel group of ATP-binding
RT cassette transporters.";
RL J. Bacteriol. 188:317-327(2006).
CC -!- FUNCTION: Part of the energy-coupling factor (ECF) transporter complex
CC CbiMNOQ involved in cobalt import. The complex confers cobalt uptake
CC upon expression in E.coli; can also transport nickel with a very low
CC affinity. {ECO:0000269|PubMed:16352848}.
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC -!- SUBUNIT: Forms an energy-coupling factor (ECF) transporter complex
CC composed of an ATP-binding protein (A component, CbiO), a transmembrane
CC protein (T component, CbiQ) and 2 possible substrate-capture proteins
CC (S components, CbiM and CbiN) of unknown stoichimetry. Expression of
CC just CbiMN in E.coli confers some cobalt uptake.
CC {ECO:0000269|PubMed:16352848}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CbiN family. {ECO:0000305}.
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DR EMBL; L12006; AAA27265.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL20926.1; -; Genomic_DNA.
DR RefSeq; NP_460967.1; NC_003197.2.
DR RefSeq; WP_000753212.1; NC_003197.2.
DR AlphaFoldDB; Q05595; -.
DR STRING; 99287.STM2022; -.
DR TCDB; 3.A.1.23.6; the atp-binding cassette (abc) superfamily.
DR PaxDb; Q05595; -.
DR EnsemblBacteria; AAL20926; AAL20926; STM2022.
DR GeneID; 1253543; -.
DR KEGG; stm:STM2022; -.
DR PATRIC; fig|99287.12.peg.2144; -.
DR HOGENOM; CLU_136197_2_0_6; -.
DR OMA; TEIEPDY; -.
DR PhylomeDB; Q05595; -.
DR BioCyc; SENT99287:STM2022-MON; -.
DR UniPathway; UPA00148; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IDA:UniProtKB.
DR GO; GO:0015087; F:cobalt ion transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006824; P:cobalt ion transport; IDA:UniProtKB.
DR HAMAP; MF_00330; CbiN; 1.
DR InterPro; IPR003705; CbiN.
DR PANTHER; PTHR38662; PTHR38662; 1.
DR Pfam; PF02553; CbiN; 1.
DR TIGRFAMs; TIGR01165; cbiN; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Cobalamin biosynthesis; Cobalt;
KW Cobalt transport; Ion transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..93
FT /note="Cobalt transport protein CbiN"
FT /id="PRO_0000134698"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 93 AA; 10269 MW; CA39465DECCA648A CRC64;
MKKTLMLLAM VVALVILPFF INHGGEYGGS DGEAESQIQA IAPQYKPWFQ PLYEPASGEI
ESLLFTLQGS LGAAVIFYIL GYCKGKQRRD DRA
