ID   CBIO_SALTY              Reviewed;         271 AA.
AC   Q05596;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2002, sequence version 2.
DT   03-AUG-2022, entry version 145.
DE   RecName: Full=Cobalt import ATP-binding protein CbiO {ECO:0000255|HAMAP-Rule:MF_01710};
DE            EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_01710};
DE   AltName: Full=Energy-coupling factor transporter ATP-binding protein CbiO {ECO:0000255|HAMAP-Rule:MF_01710};
DE            Short=ECF transporter A component CbiO {ECO:0000255|HAMAP-Rule:MF_01710};
GN   Name=cbiO {ECO:0000255|HAMAP-Rule:MF_01710}; OrderedLocusNames=STM2020;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LT2;
RX   PubMed=8501034; DOI=10.1128/jb.175.11.3303-3316.1993;
RA   Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M.;
RT   "Characterization of the cobalamin (vitamin B12) biosynthetic genes of
RT   Salmonella typhimurium.";
RL   J. Bacteriol. 175:3303-3316(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [3]
RP   FUNCTION IN COBALT TRANSPORT, SUBSTRATES, SUBUNIT, AND EXPRESSION IN
RP   E.COLI.
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=16352848; DOI=10.1128/jb.188.1.317-327.2006;
RA   Rodionov D.A., Hebbeln P., Gelfand M.S., Eitinger T.;
RT   "Comparative and functional genomic analysis of prokaryotic nickel and
RT   cobalt uptake transporters: evidence for a novel group of ATP-binding
RT   cassette transporters.";
RL   J. Bacteriol. 188:317-327(2006).
CC   -!- FUNCTION: Part of the energy-coupling factor (ECF) transporter complex
CC       CbiMNOQ involved in cobalt import. The complex confers cobalt uptake
CC       upon expression in E.coli; can also transport nickel with a very low
CC       affinity. Presumably responsible for energy coupling to the transport
CC       system. {ECO:0000269|PubMed:16352848}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SUBUNIT: Forms an energy-coupling factor (ECF) transporter complex
CC       composed of an ATP-binding protein (A component, CbiO), a transmembrane
CC       protein (T component, CbiQ) and 2 possible substrate-capture proteins
CC       (S components, CbiM and CbiN) of unknown stoichimetry. Expression of
CC       just CbiMN in E.coli confers some cobalt uptake. {ECO:0000255|HAMAP-
CC       Rule:MF_01710, ECO:0000269|PubMed:16352848}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01710}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01710}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. Cobalt importer
CC       (TC 3.A.1.18.1) family. {ECO:0000255|HAMAP-Rule:MF_01710}.
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DR   EMBL; L12006; AAA27267.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL20924.1; -; Genomic_DNA.
DR   RefSeq; NP_460965.1; NC_003197.2.
DR   RefSeq; WP_000881552.1; NC_003197.2.
DR   AlphaFoldDB; Q05596; -.
DR   SMR; Q05596; -.
DR   STRING; 99287.STM2020; -.
DR   TCDB; 3.A.1.23.6; the atp-binding cassette (abc) superfamily.
DR   PaxDb; Q05596; -.
DR   EnsemblBacteria; AAL20924; AAL20924; STM2020.
DR   GeneID; 1253541; -.
DR   KEGG; stm:STM2020; -.
DR   PATRIC; fig|99287.12.peg.2142; -.
DR   HOGENOM; CLU_000604_1_22_6; -.
DR   OMA; DIVPLYC; -.
DR   PhylomeDB; Q05596; -.
DR   BioCyc; SENT99287:STM2020-MON; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IDA:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR   GO; GO:0042626; F:ATPase-coupled transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006824; P:cobalt ion transport; IDA:UniProtKB.
DR   CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR   InterPro; IPR005876; Co_trans_ATP-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01166; cbiO; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51246; CBIO; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell inner membrane; Cell membrane; Cobalamin biosynthesis;
KW   Cobalt; Cobalt transport; Ion transport; Membrane; Nucleotide-binding;
KW   Reference proteome; Translocase; Transport.
FT   CHAIN           1..271
FT                   /note="Cobalt import ATP-binding protein CbiO"
FT                   /id="PRO_0000092059"
FT   DOMAIN          2..236
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT   BINDING         34..41
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT   CONFLICT        75
FT                   /note="L -> R (in Ref. 1; AAA27267)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        108
FT                   /note="V -> G (in Ref. 1; AAA27267)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   271 AA;  30147 MW;  D5D7F707DA76CBC6 CRC64;
     MLATSDLWFR YQNEPVLKGL NMDFSLSPVT GLVGANGCGK STLFMNLSGL LRPQKGAVLW
     QGKPLDYSKR GLLALRQQVA TVFQDPEQQI FYTDIDSDIA FSLRNLGVPE AEITRRVDEA
     LTLVDAQHFR HQPIQCLSHG QKKRVAIAGA LVLQARYLLL DEPTAGLDPA GRTQMIAIIR
     RIVAQGNHVI ISSHDIDLIY EISDAVYVLR QGQILTHGAP GEVFACTEAM EHAGLTQPWL
     VKLHTQLGLP LCKTETEFFH RMQKCAFREA S