YL016_YEAST
ID YL016_YEAST Reviewed; 1048 AA.
AC P0CF34; P14771; Q12037; Q12065; Q6B0T5; Q6WV04;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 1.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Putative truncated guanine nucleotide exchange factor SDC25;
GN Name=SDC25; Synonyms=SCD25; OrderedLocusNames=YLL016W; ORFNames=L1309;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND IDENTIFICATION OF
RP FRAMESHIFT.
RC STRAIN=ATCC 90840 / EAY235 / FY23;
RX PubMed=8810043;
RX DOI=10.1002/(sici)1097-0061(19960615)12:7<693::aid-yea956>3.0.co;2-g;
RA Miosga T., Zimmermann F.K.;
RT "Sequence analysis of the CEN12 region of Saccharomyces cerevisiae on a
RT 43.7 kb fragment of chromosome XII including an open reading frame
RT homologous to the human cystic fibrosis transmembrane conductance regulator
RT protein CFTR.";
RL Yeast 12:693-708(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9046100;
RX DOI=10.1002/(sici)1097-0061(199702)13:2<183::aid-yea65>3.0.co;2-v;
RA Purnelle B., Goffeau A.;
RT "The sequence of 32kb on the left arm of yeast chromosome XII reveals six
RT known genes, a new member of the seripauperins family and a new ABC
RT transporter homologous to the human multidrug resistance protein.";
RL Yeast 13:183-188(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC -!- INTERACTION:
CC P0CF34; Q06604: BSP1; NbExp=2; IntAct=EBI-6315249, EBI-37047;
CC -!- CAUTION: Could be the product of a pseudogene unlikely to encode a
CC functional protein. This is the C-terminal part of guanine nucleotide
CC exchange factor SDC25. Strain S288c has a frameshift in position 92,
CC which disrupts the gene coding for this protein and produces two ORFs
CC YLL016W and YLL017W. Because of that it is not part of the S.cerevisiae
CC S288c complete/reference proteome set. A contiguous sequence for SDC25
CC can be found in strain W303 (AC P0CF32). {ECO:0000305|PubMed:24374639,
CC ECO:0000305|PubMed:8810043}.
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DR EMBL; X91488; CAA62775.1; -; Genomic_DNA.
DR EMBL; X97560; CAA66161.1; -; Genomic_DNA.
DR EMBL; Z73121; CAA97461.1; -; Genomic_DNA.
DR PIR; S64758; S64758.
DR AlphaFoldDB; P0CF34; -.
DR SMR; P0CF34; -.
DR IntAct; P0CF34; 21.
DR MINT; P0CF34; -.
DR PRIDE; P0CF34; -.
DR SGD; S000003939; SDC25.
DR VEuPathDB; FungiDB:YLL016W; -.
DR InParanoid; P0CF34; -.
DR GO; GO:0016020; C:membrane; IDA:SGD.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:SGD.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007265; P:Ras protein signal transduction; IGI:SGD.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS00720; RASGEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 5: Uncertain;
KW Cell cycle; Cell division; Guanine-nucleotide releasing factor.
FT CHAIN 1..1048
FT /note="Putative truncated guanine nucleotide exchange
FT factor SDC25"
FT /id="PRO_0000393436"
FT DOMAIN 578..710
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 748..995
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT REGION 208..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 419..444
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 997..1048
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 208..223
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1010..1032
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1033..1048
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1048 AA; 121962 MW; 40A1F12F9B86F458 CRC64;
MPITSSPDLF YLNDCDVVYW YDLTRLVCHY VNLTERDLLA NEREKFLTSL DLLTAQITYV
YMLFRNLRLV EDSFKKTLKK LIYTLSRFSI NANIWFHSTL FEEREAIASQ KDPERRSPLL
QSILGTFQKF HFLLRLLHFL SNPNELTILP QLTPRFFKDS FNTISWNNPF LRKRLNQHMS
HDLPRQMIKA VAGASGIVAE NIDEIPASKQ GTSCSSETSH HSPSAPFQRR RRGTIFSNVS
GSSDESDTIW SKRKKPYPLN EETLSLVRAR KKQLDGKLKQ MIKSANEYLS NTANFSKMLN
FEMNFKTYEE VSGTIPIIDI LENLDLTIFL NLRELGDENR VFDEDVAIDD EDEEFLKHSL
SSLSYILSDY FNMKQYFHDV VVKFIIVAQH LTLEDPFVFS PMQNDLPTGY YEPMKPSSLN
LDNAKDKKNG SQNTDIQEEE DEYEPDPDSL ILFHNLINQD SDFNDLKFFN LAHVFKKSCD
DYFDVLKLAI EFVNQLILER ENLLNYAARM MKNNITELLL RGEEGYGSYD GGETAEKSDT
NAVYADSDTK DNDEWRDSQV KLPRYLQREY DSELIWGSNN RIKGGSKHAL ISYLTDNEKK
DLFFDITFLI TFRSIFTTTE FLSYLISQYN LDPPEDLCFE EYNEWVTKKL IPVKCRVVEI
MTTFFKQYWF LGYDEPDLAT LNLDYFAQVA IKENITGSVE LLKEVNQKFK HGNIQEATAP
MKTLDQQICQ DHYSGTLYST TESILAVDPV LFATQLTILE HEIYCEITIF DCLQKIWKNK
YTKSYGASPG LNEFISFANK LTNFISYSVV KEADKSKRAK LLSHFIFIAE YCRKFNNFSS
MTAIISALYS SPIYRLEKTW QAVIPQTRDL LQSLNKLMDP KKNFINYRNE LKSLHSAPCV
PFFGVYLSDL TFTDSGNPDY LVLEHGLKGV HDEKKYINFN KRSRLVDILQ EIIYFKKTHY
DFTKDRTVIE CISNSLENIP HIEKQYQLSL IIEPKPRKKV VPNSNSNNKS QEKSRDDQTD
EGKTSTKKDR FPKFQLHKTK KKAPKVSK
