ID   CBIO_SYNAS              Reviewed;         280 AA.
AC   Q2LY16;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Cobalt import ATP-binding protein CbiO {ECO:0000255|HAMAP-Rule:MF_01710};
DE            EC=7.-.-.- {ECO:0000255|HAMAP-Rule:MF_01710};
DE   AltName: Full=Energy-coupling factor transporter ATP-binding protein CbiO {ECO:0000255|HAMAP-Rule:MF_01710};
DE            Short=ECF transporter A component CbiO {ECO:0000255|HAMAP-Rule:MF_01710};
GN   Name=cbiO {ECO:0000255|HAMAP-Rule:MF_01710}; OrderedLocusNames=SYNAS_30950;
GN   ORFNames=SYN_00209;
OS   Syntrophus aciditrophicus (strain SB).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Syntrophales; Syntrophaceae;
OC   Syntrophus.
OX   NCBI_TaxID=56780;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SB;
RX   PubMed=17442750; DOI=10.1073/pnas.0610456104;
RA   McInerney M.J., Rohlin L., Mouttaki H., Kim U., Krupp R.S.,
RA   Rios-Hernandez L., Sieber J., Struchtemeyer C.G., Bhattacharyya A.,
RA   Campbell J.W., Gunsalus R.P.;
RT   "The genome of Syntrophus aciditrophicus: life at the thermodynamic limit
RT   of microbial growth.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:7600-7605(2007).
CC   -!- FUNCTION: Part of the energy-coupling factor (ECF) transporter complex
CC       CbiMNOQ involved in cobalt import. Presumably responsible for energy
CC       coupling to the transport system. {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SUBUNIT: Forms an energy-coupling factor (ECF) transporter complex
CC       composed of an ATP-binding protein (A component, CbiO), a transmembrane
CC       protein (T component, CbiQ) and 2 possible substrate-capture proteins
CC       (S components, CbiM and CbiN) of unknown stoichimetry.
CC       {ECO:0000255|HAMAP-Rule:MF_01710}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01710}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01710}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. Cobalt importer
CC       (TC 3.A.1.18.1) family. {ECO:0000255|HAMAP-Rule:MF_01710}.
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DR   EMBL; CP000252; ABC78974.1; -; Genomic_DNA.
DR   RefSeq; WP_011418988.1; NC_007759.1.
DR   AlphaFoldDB; Q2LY16; -.
DR   SMR; Q2LY16; -.
DR   STRING; 56780.SYN_00209; -.
DR   PRIDE; Q2LY16; -.
DR   EnsemblBacteria; ABC78974; ABC78974; SYN_00209.
DR   KEGG; sat:SYN_00209; -.
DR   eggNOG; COG1122; Bacteria.
DR   HOGENOM; CLU_000604_1_22_7; -.
DR   OMA; DCTKEEF; -.
DR   OrthoDB; 1713578at2; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000001933; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR   CDD; cd03225; ABC_cobalt_CbiO_domain1; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR015856; ABC_transpr_CbiO/EcfA_su.
DR   InterPro; IPR030947; EcfA_1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR04520; ECF_ATPase_1; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51246; CBIO; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell inner membrane; Cell membrane; Cobalamin biosynthesis;
KW   Cobalt; Cobalt transport; Ion transport; Membrane; Nucleotide-binding;
KW   Reference proteome; Translocase; Transport.
FT   CHAIN           1..280
FT                   /note="Cobalt import ATP-binding protein CbiO"
FT                   /id="PRO_0000288018"
FT   DOMAIN          2..236
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
FT   BINDING         36..43
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01710"
SQ   SEQUENCE   280 AA;  31164 MW;  CD6B77113F1A8962 CRC64;
     MIEVRDLRFH YQEKERAVLD GVSMTLKDGE YVALIGANGC GKTTLVHHLN ALMRPTSGVV
     SVDGLDTLDD RQVWEIRRRV GMVFQNPENQ IVGMTIEEDI AFGPGNLGLP PAEIRRRVAS
     SLELVGLESY GRRVPSALSG GEKRLVAIAG ILAMEPRYII FDEPTSYLDP ASRQRVLALI
     AGLHKRGLGI IHITHNMDDI LDVDRVLVMR EGKIVRDDRP EIVLSQGDWL RQQGLGMPAA
     TALLWRLKEM GVSVRTDILD FEDVCREIAA WKSHPVESVA