YL177_YEAST
ID YL177_YEAST Reviewed; 628 AA.
AC Q06251; D6VYI1;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Uncharacterized protein YLR177W;
GN OrderedLocusNames=YLR177W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-235, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31; SER-34 AND THR-235, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84 AND THR-235, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1550 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the PSP1 family. {ECO:0000305}.
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DR EMBL; U17246; AAB67462.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09497.1; -; Genomic_DNA.
DR PIR; S51422; S51422.
DR RefSeq; NP_013278.1; NM_001182064.1.
DR AlphaFoldDB; Q06251; -.
DR BioGRID; 31448; 44.
DR DIP; DIP-4804N; -.
DR IntAct; Q06251; 5.
DR MINT; Q06251; -.
DR STRING; 4932.YLR177W; -.
DR iPTMnet; Q06251; -.
DR MaxQB; Q06251; -.
DR PaxDb; Q06251; -.
DR PRIDE; Q06251; -.
DR EnsemblFungi; YLR177W_mRNA; YLR177W; YLR177W.
DR GeneID; 850874; -.
DR KEGG; sce:YLR177W; -.
DR SGD; S000004167; YLR177W.
DR VEuPathDB; FungiDB:YLR177W; -.
DR eggNOG; KOG4679; Eukaryota.
DR GeneTree; ENSGT00940000176361; -.
DR HOGENOM; CLU_012771_0_0_1; -.
DR InParanoid; Q06251; -.
DR OMA; DEQHANE; -.
DR BioCyc; YEAST:G3O-32302-MON; -.
DR PRO; PR:Q06251; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q06251; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR InterPro; IPR007557; PSP1_C.
DR Pfam; PF04468; PSP1; 1.
DR PROSITE; PS51411; PSP1_C; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..628
FT /note="Uncharacterized protein YLR177W"
FT /id="PRO_0000247127"
FT DOMAIN 472..565
FT /note="PSP1 C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00744"
FT REGION 133..174
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 31
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 34
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 84
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 235
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17330950,
FT ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198"
SQ SEQUENCE 628 AA; 71265 MW; 9B3575D7D9F63D26 CRC64;
MELPSINSTT SISDNQELRN YYDKLLFKNN SGKSLADLPG KMADFNDNSA AAHPRSRVDF
INGYIGFRED KQSLLGQKNT KRASFSAFAD EGRKQSEMSI NGKSPNLSLF SFEFNGTPTQ
DRKPYKQDYL NVMNTSPNNI LSPLNNSSQK YYPQKQQQQQ QQQQQQQQQS IFDPGRRSSY
ISDALIHGNA ATQQPQYSQP VYINNNPSLQ VPYTAPSEYT QQQQYSSPFN ARRNTQPVLN
LHPAAAPTND AGLAVVDGKN LTSSKELHDL YLDCGSNYFA SDKVYKFIDS IKGTLRGDNV
SASSSRIIEF LDFLKNCNLN YNPQSDAFIS TAVSNASSTG AAKSKNSTSM HLHYKPLVLV
SLKNGKLELL SKPQTATLIL KRGDLVIIDG DRGKDLVLVV EPVVDINLAL FINFLKKKIH
FDSLITNSQQ HFPNDQFIKT LVDTTNGKPV AHELNPKLYD IIELTQLIIP SKQVLRFATP
WESSTNLHNK FQDELKALHI AQLKLRSLNN NNSGGGLNIK ILNAEFQFDR KKLTFYYICQ
ERNDFRDLIK ELFKFYKTRI WLCAIPNNLS IDSKFYDSNK FEWEMYQDMM SHYSMDNTGI
VVAPELNRLK LDDFQIGVYM ELVKVLFG
