ID   CBIT_SALTY              Reviewed;         192 AA.
AC   Q05632;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating);
DE            EC=2.1.1.196;
GN   Name=cbiT; OrderedLocusNames=STM2030;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF N-TERMINUS.
RC   STRAIN=LT2;
RX   PubMed=8501034; DOI=10.1128/jb.175.11.3303-3316.1993;
RA   Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M.;
RT   "Characterization of the cobalamin (vitamin B12) biosynthetic genes of
RT   Salmonella typhimurium.";
RL   J. Bacteriol. 175:3303-3316(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-10.
RX   PubMed=1451790; DOI=10.1016/0014-5793(92)80213-z;
RA   Roessner C.A., Warren M.J., Santander P.J., Atshaves B.P., Ozaki S.,
RA   Stolowich N.J., Iida K., Scott A.I.;
RT   "Expression of 9 Salmonella typhimurium enzymes for cobinamide synthesis.
RT   Identification of the 11-methyl and 20-methyl transferases of corrin
RT   biosynthesis.";
RL   FEBS Lett. 301:73-78(1992).
RN   [4]
RP   FUNCTION.
RX   PubMed=16198574; DOI=10.1016/j.bmc.2005.08.062;
RA   Santander P.J., Kajiwara Y., Williams H.J., Scott A.I.;
RT   "Structural characterization of novel cobalt corrinoids synthesized by
RT   enzymes of the vitamin B12 anaerobic pathway.";
RL   Bioorg. Med. Chem. 14:724-731(2006).
CC   -!- FUNCTION: Catalyzes the methylation of C-15 in cobalt-precorrin-6B
CC       followed by the decarboxylation of C-12 to form cobalt-precorrin-7.
CC       {ECO:0000269|PubMed:16198574}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Co-precorrin-6B + S-adenosyl-L-methionine = Co-precorrin-7 +
CC         CO2 + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:36067,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:70791, ChEBI:CHEBI:72780; EC=2.1.1.196;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route):
CC       step 8/10.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. Bacterial-
CC       type CbiT family. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to be a precorrin-8w decarboxylase.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L12006; AAA27257.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL20934.1; -; Genomic_DNA.
DR   RefSeq; NP_460975.1; NC_003197.2.
DR   RefSeq; WP_000650985.1; NC_003197.2.
DR   AlphaFoldDB; Q05632; -.
DR   SMR; Q05632; -.
DR   STRING; 99287.STM2030; -.
DR   PaxDb; Q05632; -.
DR   EnsemblBacteria; AAL20934; AAL20934; STM2030.
DR   GeneID; 1253551; -.
DR   KEGG; stm:STM2030; -.
DR   PATRIC; fig|99287.12.peg.2152; -.
DR   HOGENOM; CLU_094143_0_0_6; -.
DR   OMA; RCKFVYA; -.
DR   PhylomeDB; Q05632; -.
DR   BioCyc; MetaCyc:MON-13226; -.
DR   BioCyc; SENT99287:STM2030-MON; -.
DR   UniPathway; UPA00148; UER00229.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0043776; F:cobalt-precorrin-6B C5-methyltransferase activity; IEA:RHEA.
DR   GO; GO:0008276; F:protein methyltransferase activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR014008; Cbl_synth_MTase_CbiT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR007848; Small_mtfrase_dom.
DR   Pfam; PF05175; MTS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   TIGRFAMs; TIGR02469; CbiT; 1.
PE   1: Evidence at protein level;
KW   Cobalamin biosynthesis; Direct protein sequencing; Methyltransferase;
KW   Reference proteome; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..192
FT                   /note="Cobalt-precorrin-6B C(15)-methyltransferase
FT                   (decarboxylating)"
FT                   /id="PRO_0000134949"
FT   BINDING         15
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
FT   BINDING         39..43
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
FT   BINDING         62
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
FT   BINDING         90
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   192 AA;  20746 MW;  70D3CE0D2DDF024B CRC64;
     MKDELFLRGE NVPMTKEAVR ALALSKLELH RASHLIDVGA GTGSVSIEAA LQFPSLQVTA
     IERNPAALRL LDENRQRFAC GNIDILPGEA PMTITGKADA VFMGGSGGHL TALIDWAMGH
     LHPGGRLVMT FILQENLHSA LAHLAHIGAC RMDCVQLQLS SLTPLGAGHY FKPNNPVFVI
     ACQKEENHVR DI