ID   YL225_YEAST             Reviewed;         407 AA.
AC   Q05948; D6VYM5;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Uncharacterized SVF1-like protein YLR225C;
GN   OrderedLocusNames=YLR225C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA   Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA   Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA   Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA   Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA   Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA   Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA   Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA   Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA   Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA   Zollner A., Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-22, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22106047; DOI=10.1002/pmic.201100166;
RA   Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT   "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL   Proteomics 12:236-240(2012).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 3990 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the SVF1 family. {ECO:0000305}.
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DR   EMBL; U19027; AAB67413.1; -; Genomic_DNA.
DR   EMBL; BK006945; DAA09541.1; -; Genomic_DNA.
DR   PIR; S51448; S51448.
DR   RefSeq; NP_013326.1; NM_001182112.1.
DR   AlphaFoldDB; Q05948; -.
DR   BioGRID; 31492; 99.
DR   DIP; DIP-4343N; -.
DR   iPTMnet; Q05948; -.
DR   MaxQB; Q05948; -.
DR   PaxDb; Q05948; -.
DR   PRIDE; Q05948; -.
DR   EnsemblFungi; YLR225C_mRNA; YLR225C; YLR225C.
DR   GeneID; 850922; -.
DR   KEGG; sce:YLR225C; -.
DR   SGD; S000004215; YLR225C.
DR   VEuPathDB; FungiDB:YLR225C; -.
DR   eggNOG; ENOG502QQBB; Eukaryota.
DR   GeneTree; ENSGT00940000176680; -.
DR   HOGENOM; CLU_030205_0_0_1; -.
DR   InParanoid; Q05948; -.
DR   OMA; IDIWESF; -.
DR   BioCyc; YEAST:G3O-32339-MON; -.
DR   PRO; PR:Q05948; -.
DR   Proteomes; UP000002311; Chromosome XII.
DR   RNAct; Q05948; protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0006979; P:response to oxidative stress; IEA:InterPro.
DR   InterPro; IPR033394; Svf1-like_C.
DR   InterPro; IPR013931; Svf1-like_N.
DR   Pfam; PF08622; Svf1; 1.
DR   Pfam; PF17187; Svf1_C; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Isopeptide bond; Reference proteome; Ubl conjugation.
FT   CHAIN           1..407
FT                   /note="Uncharacterized SVF1-like protein YLR225C"
FT                   /id="PRO_0000247175"
FT   CROSSLNK        22
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ubiquitin)"
FT                   /evidence="ECO:0007744|PubMed:22106047"
SQ   SEQUENCE   407 AA;  46423 MW;  B251F1AAE421DBDA CRC64;
     MAVAIKKEKT KFAPVKEVLS EKDHANYTKF QDTSKLEWFC RTSNHKKFKS HSLLKAVRNP
     TETRIETQTL YFTDLTNGKC GLIQLLYSSV MGGIYKGFQL NFKIFKASSE ENSEEDIDIW
     ESFKIDNIKD FDTLKVESDN VTFHFVPLEN SSSSGFAQLL IKIDIPKGST SCLLKDLKVD
     ITVNLQEGFI INPDGSNYYL DKSISLEELA KRDSSSTSRK MIRHVFVPRG FCNGTISYKK
     NDKPVKLDLK DTPMLYLDAV QGLIPNKAAS KWNFLCFNGE KRSMMCIEFT TTKEYGSTTV
     TIWAVSDKDK ILEVGSSVND HAVKFPSTKE DKQNGWKYPT SISFPRGFEE SNLRLVNRYD
     IMSELPAFIR SIAENLANMK PFIYQFCQKS KFDDDEGVSI IESTFIN