YL278_YEAST
ID YL278_YEAST Reviewed; 1341 AA.
AC Q05854; D6VYS5;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Uncharacterized transcriptional regulatory protein YLR278C;
GN OrderedLocusNames=YLR278C; ORFNames=L8003.10;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1143, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 1080 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U17243; AAB67326.1; -; Genomic_DNA.
DR EMBL; U17245; AAB67370.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09591.1; -; Genomic_DNA.
DR PIR; S50366; S50366.
DR RefSeq; NP_013380.1; NM_001182165.1.
DR AlphaFoldDB; Q05854; -.
DR SMR; Q05854; -.
DR BioGRID; 31546; 160.
DR DIP; DIP-7433N; -.
DR IntAct; Q05854; 4.
DR MINT; Q05854; -.
DR STRING; 4932.YLR278C; -.
DR iPTMnet; Q05854; -.
DR MaxQB; Q05854; -.
DR PaxDb; Q05854; -.
DR PRIDE; Q05854; -.
DR EnsemblFungi; YLR278C_mRNA; YLR278C; YLR278C.
DR GeneID; 850984; -.
DR KEGG; sce:YLR278C; -.
DR SGD; S000004268; YLR278C.
DR VEuPathDB; FungiDB:YLR278C; -.
DR eggNOG; ENOG502QS9Q; Eukaryota.
DR GeneTree; ENSGT00940000176304; -.
DR HOGENOM; CLU_004038_1_0_1; -.
DR InParanoid; Q05854; -.
DR OMA; AHDMINN; -.
DR BioCyc; YEAST:G3O-32377-MON; -.
DR PRO; PR:Q05854; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q05854; protein.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR007219; Transcription_factor_dom_fun.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF04082; Fungal_trans; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00906; Fungal_trans; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation; Zinc.
FT CHAIN 1..1341
FT /note="Uncharacterized transcriptional regulatory protein
FT YLR278C"
FT /id="PRO_0000115003"
FT DNA_BIND 41..68
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 72..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 770..804
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 864..971
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1031..1116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1220..1240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 72..91
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 770..797
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1143
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
SQ SEQUENCE 1341 AA; 151279 MW; 94201F3964500EEF CRC64;
MGRPRKNVSQ EKIQQLKREL ELAGNRTDVL LQDKKGRSRS CLLCRRRKQR CDHKLPSCTA
CLKAGIKCVQ PSKYSSSTSN SNTNNNTPTA GTVPPTPHPV IKRELQDSSI GAGAGAATSL
NDMTIIKPIS TSNSNVDAGD ANEFRKTIKS VTTNSNPNLM RQDKDQYTIF LEKKLKSLET
LLDLSPGCNQ YNYELSQYKK VSHLFSNNTS DYSRPNSSNM VILPLPSPSN KPLENTNNNG
SNVNAATNDT SASTNNINNN NAICQSASLL NDPLETLDFT KCIFAKYNLK KEFLMYDPIF
ELNEKLSRSF LDTFFTRLQF KYPILDEQEI YTFYDHYLHN KILIPPSSPA TSSAAPPSNS
HSYSEIEFHF LSGRMWLVFS ISAYLLMTTG KYKGFPPHRY FSTAIRHITK CGLHLNYVQQ
IELLTLLVLY IIRTDRDSLI LYDIIKDVMG ISKKKLHLNQ WYPNDPFANK KLRLFWCVYL
LERMICVAVG KPYTIKESEI NLPLFNNDSF YTKGVHAAAP STNDHGVQFI NQSLKLRRIE
SQFVETLQLL KNDSRSVKQS IDQLPLVRKF FEDLEVWRKS YSTLDVKNFE NETLKLYYYR
SVRLLIQPYL EFFAPEDRLF RECQAAAGQI CQLYKIFHQK TLNGHSTPAV HTVFVAGVTL
IYCMWLARNF DDQRRKKLGD ASKHTRPLIS ASLFSTMDDL RACSVCLYVM TERSNFARTF
RDTFDQLMNA TVGNLIERCG PDSSELIFMA SSVAKRTEPK NINDEANKAI SSGDTLHDSN
SANAANLSNS NDKNISHNGG MPPAVARIFG KGQAEEHAGF VENSQVDLAE QEKFKKKQGV
LEKTSVPKSL AHLLTKMDDR SRISNSSMSY TTSSSSSSSS SSSSSTLSFP SSQEKNLKIN
VNNDNNGMTI SSVNREHNNN HNNNNDNNNN NNNNNNNSNN NNNVNNNDNE SNSRSTTNNS
CNNGNNSQYV RNNNVTMEND VERPIQDQYI VKKPTNQTEF DWQVFQQQAF LQQQLAQHNL
QAYLSSLNTD TMTNRSPSKS SSISTASSHS DPIPIAMTQS PTPYPQTSNM LPQQHVSRPL
PQQQREQPQQ HITSPQRFSE SNFTNQLNNG MINSNPLQSA IFSNHTSENK QLRDVEESNF
STSPLRADYG NNIISSIPAS FTSNSIPVSV KQARNGSSSG DILFSNGAHD MINNISTWTN
NSVLDALNSK SILQTIFPQS QEPSSLSMDK QQQQHQQQNM CSENNVTANN FQQTQNDPSY
NRNLFMMSNQ EGVQYNLDET EKNGPKTQVE ANTSANLHFD NVIPTVTNAD IRKKRSNWDN
MMTSGPVEDF WTINDDYGFL T
