YL297_YEAST
ID YL297_YEAST Reviewed; 129 AA.
AC Q05899; D6VYU1;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Uncharacterized vacuolar protein YLR297W;
GN OrderedLocusNames=YLR297W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP INDUCTION.
RX PubMed=15878181; DOI=10.1016/j.mrfmmm.2005.02.005;
RA Caba E., Dickinson D.A., Warnes G.R., Aubrecht J.;
RT "Differentiating mechanisms of toxicity using global gene expression
RT analysis in Saccharomyces cerevisiae.";
RL Mutat. Res. 575:34-46(2005).
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}.
CC -!- INDUCTION: By the genotoxic agents methyl methanesulfonate (MMS),
CC bleomycin and cisplatin. {ECO:0000269|PubMed:15878181}.
CC -!- MISCELLANEOUS: Present with 922 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U17243; AAB67342.1; -; Genomic_DNA.
DR EMBL; AY558216; AAS56542.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09607.1; -; Genomic_DNA.
DR PIR; S50381; S50381.
DR RefSeq; NP_013400.1; NM_001182185.1.
DR AlphaFoldDB; Q05899; -.
DR SMR; Q05899; -.
DR BioGRID; 31561; 62.
DR DIP; DIP-4684N; -.
DR IntAct; Q05899; 3.
DR MINT; Q05899; -.
DR STRING; 4932.YLR297W; -.
DR iPTMnet; Q05899; -.
DR PaxDb; Q05899; -.
DR EnsemblFungi; YLR297W_mRNA; YLR297W; YLR297W.
DR GeneID; 851004; -.
DR KEGG; sce:YLR297W; -.
DR SGD; S000004288; YLR297W.
DR VEuPathDB; FungiDB:YLR297W; -.
DR HOGENOM; CLU_1950497_0_0_1; -.
DR OMA; MCGDHHS; -.
DR BioCyc; YEAST:G3O-32390-MON; -.
DR ChiTaRS; YLR297W; yeast.
DR PRO; PR:Q05899; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q05899; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0000329; C:fungal-type vacuole membrane; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix; Vacuole.
FT CHAIN 1..129
FT /note="Uncharacterized vacuolar protein YLR297W"
FT /id="PRO_0000247161"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 109..129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 113..129
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 129 AA; 13947 MW; B5C03B420AD4C51A CRC64;
MVEGDFVDEQ SNIALLSSKS MCGDHHSVKN SIGDEIFKLL TKILNSDEKA SGDVHTLVSG
TPDLSNFNLD NEPLENILAV FIISFIIVVV GVLLLGLIGM IFISLRSGSS NDKKLQSNDE
EKQALAEKA
