ID   YL540_MIMIV             Reviewed;         695 AA.
AC   Q5UQ96;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Putative ATP-dependent RNA helicase L540;
DE            EC=3.6.4.13;
GN   OrderedLocusNames=MIMI_L540;
OS   Acanthamoeba polyphaga mimivirus (APMV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes;
OC   Imitervirales; Mimiviridae; Mimivirus.
OX   NCBI_TaxID=212035;
OH   NCBI_TaxID=5757; Acanthamoeba polyphaga (Amoeba).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Rowbotham-Bradford;
RX   PubMed=15486256; DOI=10.1126/science.1101485;
RA   Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H.,
RA   La Scola B., Susan M., Claverie J.-M.;
RT   "The 1.2-megabase genome sequence of Mimivirus.";
RL   Science 306:1344-1350(2004).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=16971431; DOI=10.1128/jvi.00940-06;
RA   Renesto P., Abergel C., Decloquement P., Moinier D., Azza S., Ogata H.,
RA   Fourquet P., Gorvel J.-P., Claverie J.-M., Raoult D.;
RT   "Mimivirus giant particles incorporate a large fraction of anonymous and
RT   unique gene products.";
RL   J. Virol. 80:11678-11685(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:16971431}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AY653733; AAV50804.1; -; Genomic_DNA.
DR   RefSeq; YP_003987055.1; NC_014649.1.
DR   SMR; Q5UQ96; -.
DR   GeneID; 9925174; -.
DR   KEGG; vg:9925174; -.
DR   Proteomes; UP000001134; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR007502; Helicase-assoc_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF04408; HA2; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW   Virion.
FT   CHAIN           1..695
FT                   /note="Putative ATP-dependent RNA helicase L540"
FT                   /id="PRO_0000247281"
FT   DOMAIN          53..219
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          247..434
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   MOTIF           164..167
FT                   /note="DEAH box"
FT   BINDING         66..73
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ   SEQUENCE   695 AA;  79556 MW;  12A49B7C1F544616 CRC64;
     MASNLTFEDK IGILDPKGLR PNPLNNEPYS DDYKKLAMVW STYPAYSAAD RVLSALENYQ
     LVFVTSSTGS GKSVLIPKLA LHYTNYNGRV VMTLPKRIIT LSAAIFSAKV SDVKLGESIG
     YAYKGSDKSM YNDQNKIIYV TDGIFVMEYV RDPLLSKFNV VIIDEAHERR IQIDLILLFL
     RTLLQSGNRP DLKVIIMSAT IDTDKYQKYF NSVDSTVIDI AGQPNHPIET HFMDKPVTSY
     MKEGLELIED LIHQQIKKDM LFFITTSNEA LQLCRSIRPQ YPRVYCVEVY SDMDKNLKQY
     AESRDKYLEL GNYDQKLVMA TNVAESSLTI DGLVYVIDSG YELSSRFDPE CYGQILEKKF
     VSKAQALQRR GRVGRTEPGV CYHLLTKQQF DGLADYPTPD ILRQDITMDL IKIIQVSPNK
     TYAEGINMMN QLMDPPLRSH INATRNLFDL YNVVDDNGIL TQVGIVATQF SSLPLNRILF
     LIYAFELQCA REASIIVAMT EFLNGRVTNL FYKSDTICES NCEKQAANLL LEKLIQKRGD
     HFTYLKIYQE FSKSTDQKSW ARKYGVRLDT INNIERTANQ YFYRILNLLR KPRLPNNKNT
     LIDTSIDTPM DIQSRISSTD TKTNLLNALK KSHQHLTASK LKPTYSKENI TGKISRDSVL
     NQIYKKNEIS KKKIIYDELS NINGKWEFRT VTIIS