YL85_SCHPO
ID YL85_SCHPO Reviewed; 450 AA.
AC Q9UTB3;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Putative tRNA pseudouridine synthase C25B8.05;
DE EC=5.4.99.-;
GN ORFNames=SPAC25B8.05;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in tRNA = a pseudouridine in tRNA;
CC Xref=Rhea:RHEA:54572, Rhea:RHEA-COMP:13339, Rhea:RHEA-COMP:13934,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000305}.
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DR EMBL; CU329670; CAB61771.1; -; Genomic_DNA.
DR PIR; T50192; T50192.
DR RefSeq; NP_594465.1; NM_001019894.2.
DR AlphaFoldDB; Q9UTB3; -.
DR SMR; Q9UTB3; -.
DR BioGRID; 278075; 64.
DR STRING; 4896.SPAC25B8.05.1; -.
DR iPTMnet; Q9UTB3; -.
DR MaxQB; Q9UTB3; -.
DR PaxDb; Q9UTB3; -.
DR PRIDE; Q9UTB3; -.
DR EnsemblFungi; SPAC25B8.05.1; SPAC25B8.05.1:pep; SPAC25B8.05.
DR GeneID; 2541578; -.
DR KEGG; spo:SPAC25B8.05; -.
DR PomBase; SPAC25B8.05; -.
DR VEuPathDB; FungiDB:SPAC25B8.05; -.
DR eggNOG; KOG2554; Eukaryota.
DR HOGENOM; CLU_014673_2_0_1; -.
DR InParanoid; Q9UTB3; -.
DR OMA; ARFNCKS; -.
DR PhylomeDB; Q9UTB3; -.
DR PRO; PR:Q9UTB3; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0009982; F:pseudouridine synthase activity; ISO:PomBase.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:1990481; P:mRNA pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; ISO:PomBase.
DR CDD; cd02569; PseudoU_synth_ScPus3; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR Gene3D; 3.30.70.660; -; 1.
DR HAMAP; MF_00171; TruA; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR041707; Pus3-like.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR PANTHER; PTHR11142; PTHR11142; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00071; hisT_truA; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Isomerase; Lyase; Nucleus; Reference proteome; tRNA processing.
FT CHAIN 1..450
FT /note="Putative tRNA pseudouridine synthase C25B8.05"
FT /id="PRO_0000316611"
FT ACT_SITE 149
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 208
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 450 AA; 51604 MW; 8576E531F9AC2B92 CRC64;
MPASKYAGWS RDQLISRILE LDGKLQKNTN SLPNKEIEGK NEVIKNKNKL DNGNEIEPQH
LSQELVLQHA LQDASSLPKL KPVRPFQLEK TVFVAFKFAY CGWNYNGLAY QLEPTPLPTV
EGKVFEALLR AHLITDPSSC SFSRCGRTDK GVSAMGQVIS LNVRHSERRP IIYCDVLNRL
LPADIRITGY AFPPEGFDAR FSCKQRHYKY LFTKDYPGGS LDIDRMNEAA ALYLGEHDFR
NFCKIDASKQ ITNYHRRILS SKVICIDPST GLYAFDLQGT AFLWHQVRCM MAILFLIGQK
LEPASLINDL LDIKKVPTKP IYDMASEYPL ILYDCDFDNI EWTLPTDSPT TAPRIAKHTY
ETIYHQWHSL RIREQIASFM LDIADHNVKR YGKSEESSST MNVGEGLLKR TKKYIPILSR
QRQESVEVVN ERYKRKKGLK DSDVKSENIK
