CBL10_ARATH
ID CBL10_ARATH Reviewed; 672 AA.
AC Q9LJU0;
DT 22-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=COBRA-like protein 10 {ECO:0000303|PubMed:12376623};
DE Flags: Precursor;
GN Name=COBL10 {ECO:0000303|PubMed:12376623};
GN OrderedLocusNames=At3g20580 {ECO:0000312|Araport:AT3G20580};
GN ORFNames=K10D20.11 {ECO:0000312|EMBL:BAB01166.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=12376623; DOI=10.1104/pp.007468;
RA Roudier F., Schindelman G., DeSalle R., Benfey P.N.;
RT "The COBRA family of putative GPI-anchored proteins in Arabidopsis. A new
RT fellowship in expansion.";
RL Plant Physiol. 130:538-548(2002).
RN [6]
RP FUNCTION, MUTAGENESIS OF 664-ILE--ASP-672, DISRUPTION PHENOTYPE,
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=23384085; DOI=10.1111/tpj.12139;
RA Li S., Ge F.-R., Xu M., Zhao X.-Y., Huang G.-Q., Zhou L.-Z., Wang J.-G.,
RA Kombrink A., McCormick S., Zhang X.S., Zhang Y.;
RT "Arabidopsis COBRA-LIKE 10, a GPI-anchored protein, mediates directional
RT growth of pollen tubes.";
RL Plant J. 74:486-497(2013).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, GPI-ANCHOR, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=24963069; DOI=10.1104/pp.114.236133;
RA Dai X.R., Gao X.-Q., Chen G.H., Tang L.L., Wang H., Zhang X.S.;
RT "ABNORMAL POLLEN TUBE GUIDANCE1, an endoplasmic reticulum-localized
RT mannosyltransferase homolog of GLYCOSYLPHOSPHATIDYLINOSITOL10 in yeast and
RT PHOSPHATIDYLINOSITOL GLYCAN ANCHOR BIOSYNTHESIS B in human, is required for
RT Arabidopsis pollen tube micropylar guidance and embryo development.";
RL Plant Physiol. 165:1544-1556(2014).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=27872247; DOI=10.1104/pp.16.01719;
RA Gao H., Zhang Y., Wang W., Zhao K., Liu C., Bai L., Li R., Guo Y.;
RT "Two membrane-anchored aspartic proteases contribute to pollen and ovule
RT development.";
RL Plant Physiol. 173:219-239(2017).
CC -!- FUNCTION: Involved in the deposition of apical pectin cap and cellulose
CC microfibrils in pollen tubes (PubMed:23384085). Not essential for
CC pollen development, hydration or germination, but required for pollen
CC tubes growth in the female transmitting tract of pistil and toward
CC micropyles, via the perception of ovule guidance cues (PubMed:23384085,
CC PubMed:24963069, PubMed:27872247). {ECO:0000269|PubMed:23384085,
CC ECO:0000269|PubMed:24963069, ECO:0000269|PubMed:27872247}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23384085,
CC ECO:0000269|PubMed:24963069, ECO:0000269|PubMed:27872247}; Lipid-
CC anchor, GPI-anchor {ECO:0000269|PubMed:23384085,
CC ECO:0000269|PubMed:24963069}. Cytoplasm {ECO:0000269|PubMed:24963069,
CC ECO:0000269|PubMed:27872247}. Vesicle {ECO:0000269|PubMed:23384085}.
CC Note=Localization to the plasma membrane is dependent of the GPI-anchor
CC at Ser-646 (PubMed:24963069, PubMed:23384085). Localized at the apical
CC plasma membrane of pollen tubes (PubMed:24963069, PubMed:23384085).
CC Colocalizes with A36 and A39 at the plasma membrane and in the
CC cytoplasm in the growing pollen tubes (PubMed:27872247). In the
CC cytoplasm, observed in motile punctate vesicles (PubMed:23384085).
CC {ECO:0000269|PubMed:23384085, ECO:0000269|PubMed:24963069,
CC ECO:0000269|PubMed:27872247}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves, flowers and
CC siliques (PubMed:12376623). Specific expression in the pollen tube
CC (PubMed:23384085). {ECO:0000269|PubMed:12376623,
CC ECO:0000269|PubMed:23384085}.
CC -!- PTM: The GPI-anchor attachment at Ser-646 requires APTG1.
CC {ECO:0000269|PubMed:24963069}.
CC -!- DISRUPTION PHENOTYPE: Gametophytic male sterility due to reduced pollen
CC tube growth and compromised directional ovular guidance cues sensing in
CC the female transmitting tract, thus leading to an abnormal pollen tubes
CC guidance growth toward micropyles (PubMed:24963069, PubMed:27872247,
CC PubMed:23384085). Normal pollen development, hydration and germination
CC (PubMed:23384085). Disrupted apical pectin cap and cellulose
CC microfibrils deposition in pollen tubes resulting in an abnormal cell
CC wall organization (PubMed:23384085). {ECO:0000269|PubMed:23384085,
CC ECO:0000269|PubMed:24963069, ECO:0000269|PubMed:27872247}.
CC -!- SIMILARITY: Belongs to the COBRA family. {ECO:0000305}.
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DR EMBL; AP000410; BAB01166.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76400.1; -; Genomic_DNA.
DR EMBL; AK118605; BAC43204.1; -; mRNA.
DR EMBL; BT005952; AAO64887.1; -; mRNA.
DR RefSeq; NP_188694.2; NM_112950.3.
DR AlphaFoldDB; Q9LJU0; -.
DR STRING; 3702.AT3G20580.1; -.
DR PaxDb; Q9LJU0; -.
DR PRIDE; Q9LJU0; -.
DR ProteomicsDB; 223954; -.
DR EnsemblPlants; AT3G20580.1; AT3G20580.1; AT3G20580.
DR GeneID; 821605; -.
DR Gramene; AT3G20580.1; AT3G20580.1; AT3G20580.
DR KEGG; ath:AT3G20580; -.
DR Araport; AT3G20580; -.
DR TAIR; locus:2085785; AT3G20580.
DR eggNOG; ENOG502QUPM; Eukaryota.
DR HOGENOM; CLU_420019_0_0_1; -.
DR InParanoid; Q9LJU0; -.
DR OMA; MHVCCKR; -.
DR OrthoDB; 309219at2759; -.
DR PhylomeDB; Q9LJU0; -.
DR PRO; PR:Q9LJU0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LJU0; baseline and differential.
DR Genevisible; Q9LJU0; AT.
DR GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR GO; GO:0016324; C:apical plasma membrane; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0090406; C:pollen tube; IDA:UniProtKB.
DR GO; GO:0031982; C:vesicle; IDA:UniProtKB.
DR GO; GO:0071555; P:cell wall organization; IMP:UniProtKB.
DR GO; GO:0010215; P:cellulose microfibril organization; IEA:InterPro.
DR GO; GO:0009860; P:pollen tube growth; IMP:UniProtKB.
DR GO; GO:0010183; P:pollen tube guidance; IMP:UniProtKB.
DR InterPro; IPR006918; COBRA_pln.
DR Pfam; PF04833; COBRA; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cytoplasm; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Signal.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..646
FT /note="COBRA-like protein 10"
FT /id="PRO_0000005587"
FT PROPEP 647..672
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000005588"
FT DOMAIN 502..607
FT /note="CBM2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01135"
FT MOTIF 664..672
FT /note="Required for processing by the PIG complex, a
FT critical step for apical plasma membrane localization in
FT pollen tubes"
FT /evidence="ECO:0000269|PubMed:23384085"
FT LIPID 646
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 79
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 135
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 264
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 328
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 339
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 368
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 422
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 442
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 483
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 562
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 570
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 589
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT MUTAGEN 664..672
FT /note="IAAFALMMD->NNNNNNNNN: Lost apical plasma membrane
FT localization in pollen tubes."
FT /evidence="ECO:0000269|PubMed:23384085"
SQ SEQUENCE 672 AA; 74670 MW; 9E106A2A0FEE6353 CRC64;
MRAIDVKTGM KIPWDVRYSL SLFIFLSSIL FLSNGQDYGM PGEDGGGGAE PPPEMAHCNG
IFMSYNFGSR EREYPHVKNV TAQSWAFKST AMIVNAGREE LKGWQMFIGF RHKELIVSAT
GATPMDGDYP LDASNGTTFV GSPNMDLKTS IETAGDFTQI SANIEITGTL FGVSKAVTPM
PRTIKLTNDG WECPAAKRKG GSMHVCCKRN PKIKNKIGLK TKFAPRRYGD LNIVYDVLQS
FDSNYLAQVT IDNDNPLGRL DRWNLTFEWM RGEFINTMRG AYTHKKDPSE CLYSKAGQYY
KDLDFSQVMN CQRKPAISDL PPEKKEDNMT GKLPFCCKNG TLLPPIMDPS KSRSMFQLQV
FKLPPDLNRT ALYPPQHWKI DGVLNPQYKC GPPVRVDPSQ FPDPSGLLAV TYAISSWQVV
CNITKPKAQA SRCCVSFSAF YNNSAVPCNT CACGCNDIDT DTCNANSNPL LLPPDALLVP
FDNRTLKAKA WAKQNHMPVP KKLPCPDNCG VSINWHVSTD YKNGWTARLT VFNWRDFAFE
DWFVAIDMGK AGPGYENVYS FNGTRVPPSN RTVIFQGLPG MNYLVGQVNG TNPLRDPPVP
GKQQSVISFT KKNIKGLNIP EGDGFPTKLF FNGEECALPK HFPKKSSGHR RGISVSMSFV
FATIAAFALM MD
