YL8H_SCHPO
ID YL8H_SCHPO Reviewed; 295 AA.
AC Q9UTA3;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Probable intramembrane protease C25B8.17;
DE EC=3.4.23.-;
GN ORFNames=SPAC25B8.17;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:16823372}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16823372}. Golgi apparatus membrane
CC {ECO:0000269|PubMed:16823372}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16823372}.
CC -!- DOMAIN: The PAL motif is required for normal active site conformation.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase A22B family. {ECO:0000305}.
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DR EMBL; CU329670; CAB61783.1; -; Genomic_DNA.
DR PIR; T50204; T50204.
DR RefSeq; NP_594477.1; NM_001019906.2.
DR AlphaFoldDB; Q9UTA3; -.
DR BioGRID; 279165; 8.
DR STRING; 4896.SPAC25B8.17.1; -.
DR MEROPS; A22.A11; -.
DR MaxQB; Q9UTA3; -.
DR PaxDb; Q9UTA3; -.
DR EnsemblFungi; SPAC25B8.17.1; SPAC25B8.17.1:pep; SPAC25B8.17.
DR GeneID; 2542712; -.
DR KEGG; spo:SPAC25B8.17; -.
DR PomBase; SPAC25B8.17; -.
DR VEuPathDB; FungiDB:SPAC25B8.17; -.
DR eggNOG; KOG2443; Eukaryota.
DR HOGENOM; CLU_023799_0_0_1; -.
DR InParanoid; Q9UTA3; -.
DR OMA; LFSYEDH; -.
DR PhylomeDB; Q9UTA3; -.
DR PRO; PR:Q9UTA3; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:PomBase.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0071458; C:integral component of cytoplasmic side of endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0071556; C:integral component of lumenal side of endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; ISO:PomBase.
DR GO; GO:1990578; C:perinuclear endoplasmic reticulum membrane; ISO:PomBase.
DR GO; GO:0042500; F:aspartic endopeptidase activity, intramembrane cleaving; ISO:PomBase.
DR GO; GO:0033619; P:membrane protein proteolysis; IBA:GO_Central.
DR GO; GO:0006465; P:signal peptide processing; IBA:GO_Central.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; ISO:PomBase.
DR InterPro; IPR007369; Peptidase_A22B_SPP.
DR InterPro; IPR006639; Preselin/SPP.
DR PANTHER; PTHR12174; PTHR12174; 1.
DR Pfam; PF04258; Peptidase_A22B; 1.
DR SMART; SM00730; PSN; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Golgi apparatus; Hydrolase; Membrane; Protease;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..295
FT /note="Probable intramembrane protease C25B8.17"
FT /id="PRO_0000316621"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 22..35
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 57..63
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 85..89
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 107..111
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 131..139
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..183
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 205..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 245..249
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..268
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 269..295
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOTIF 249..251
FT /note="PAL"
FT ACT_SITE 149
FT /evidence="ECO:0000250"
FT ACT_SITE 190
FT /evidence="ECO:0000250"
SQ SEQUENCE 295 AA; 33234 MW; 46657590EB20F1F9 CRC64;
MEGVILASSA LFTVYIGAKW SAQEEEPEEK QLINKRLAVL FPIFGGVTLV LMYLALRYLS
KEYIQLILQG YASLASIICF VRSFNPKTTF GKITATMSSI AIALFYFKTK HWMASNILAW
ALAANSISIM RIDSYNTGAL LLGALFFYDI YFVFGTEVMV TVATGIDIPA KYVLPQFKNP
TRLSMLGLGD IVMPGLMLAL MYRFDLHYYI NSTSQPKKHS TYFRNTFIAY GLGLGVTNFA
LYYFKAAQPA LLYLSPACIV APLLTAWYRD ELKTLFSFRS ETEDETDEQD KCKST
