YLAJ_BACSU
ID YLAJ_BACSU Reviewed; 209 AA.
AC O07634;
DT 30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Probable spore germination lipoprotein YlaJ {ECO:0000305};
DE Flags: Precursor;
GN Name=ylaJ; OrderedLocusNames=BSU14800;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Purnelle B., Presecan E., Glaser P., Richou A., Danchin A., Goffeau A.;
RT "Bacillus subtilis chromosomal region downstream nprE.";
RL Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=26731423; DOI=10.1021/acs.jproteome.5b00976;
RA Zheng L., Abhyankar W., Ouwerling N., Dekker H.L., van Veen H.,
RA van der Wel N.N., Roseboom W., de Koning L.J., Brul S., de Koster C.G.;
RT "Bacillus subtilis spore inner membrane proteome.";
RL J. Proteome Res. 15:585-594(2016).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=28333204; DOI=10.1093/femsle/fnx047;
RA Johnson C.L., Moir A.;
RT "Proteins YlaJ and YhcN contribute to the efficiency of spore germination
RT in Bacillus subtilis.";
RL FEMS Microbiol. Lett. 364:0-0(2017).
CC -!- FUNCTION: Probably contributes, directly or indirectly, to early events
CC in germination. {ECO:0000269|PubMed:28333204}.
CC -!- SUBCELLULAR LOCATION: Forespore inner membrane
CC {ECO:0000269|PubMed:26731423}; Lipid-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00303}.
CC -!- DISRUPTION PHENOTYPE: Mutant shows a reduced rate of spore germination
CC in L-alanine. {ECO:0000269|PubMed:28333204}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z97025; CAB09715.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13353.1; -; Genomic_DNA.
DR PIR; H69872; H69872.
DR RefSeq; NP_389363.1; NC_000964.3.
DR RefSeq; WP_003232269.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; O07634; -.
DR SMR; O07634; -.
DR STRING; 224308.BSU14800; -.
DR PaxDb; O07634; -.
DR PRIDE; O07634; -.
DR EnsemblBacteria; CAB13353; CAB13353; BSU_14800.
DR GeneID; 935926; -.
DR KEGG; bsu:BSU14800; -.
DR PATRIC; fig|224308.179.peg.1614; -.
DR eggNOG; ENOG5030RSQ; Bacteria.
DR OMA; RVGTIKY; -.
DR BioCyc; BSUB:BSU14800-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:InterPro.
DR InterPro; IPR014247; Spore_lipoprot_YhcN/YlaJ.
DR InterPro; IPR019076; Spore_lipoprot_YhcN/YlaJ-like.
DR Pfam; PF09580; Spore_YhcN_YlaJ; 1.
DR TIGRFAMs; TIGR02898; spore_YhcN_YlaJ; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Germination; Lipoprotein; Membrane; Palmitate; Reference proteome; Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 17..209
FT /note="Probable spore germination lipoprotein YlaJ"
FT /id="PRO_0000018063"
FT REGION 26..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 165..209
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..54
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 186..209
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 17
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 17
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ SEQUENCE 209 AA; 23384 MW; B09FD421AD42CB09 CRC64;
MRILFIIIQL TLILSACAYQ QGKQGQNVEK ESTRQQETKP IHVKDTTQET KDNGTRTDIA
KHLVSVAEKN PDVKDATAVV LGGYAVVGID VDDTLDRSKV ETIKYSVAQA LKNDRYGANA
VVIADPDTVS RLREMSREIS EGHPVTGILD ELAAIVGRVL PEVPNDVIDN EDEPQTKQQN
DQLNRKQQQE MEKEQNDQSD HHMKKNNND
