ID   YLJE_LACLA              Reviewed;         460 AA.
AC   Q9CGB9;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Uncharacterized RNA methyltransferase YljE;
DE            EC=2.1.1.-;
GN   Name=yljE; OrderedLocusNames=LL1178; ORFNames=L193873;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01024}.
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DR   EMBL; AE005176; AAK05276.1; -; Genomic_DNA.
DR   PIR; B86772; B86772.
DR   RefSeq; NP_267334.1; NC_002662.1.
DR   AlphaFoldDB; Q9CGB9; -.
DR   SMR; Q9CGB9; -.
DR   STRING; 272623.L193873; -.
DR   PaxDb; Q9CGB9; -.
DR   PRIDE; Q9CGB9; -.
DR   EnsemblBacteria; AAK05276; AAK05276; L193873.
DR   KEGG; lla:L193873; -.
DR   PATRIC; fig|272623.7.peg.1262; -.
DR   eggNOG; COG2265; Bacteria.
DR   HOGENOM; CLU_014689_7_0_9; -.
DR   OMA; FYAGDMK; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR030391; MeTrfase_TrmA_CS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   PANTHER; PTHR11061; PTHR11061; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
DR   PROSITE; PS01231; TRMA_2; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..460
FT                   /note="Uncharacterized RNA methyltransferase YljE"
FT                   /id="PRO_0000161987"
FT   DOMAIN          9..67
FT                   /note="TRAM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00208"
FT   ACT_SITE        417
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         292
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         321
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         342
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
FT   BINDING         390
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   460 AA;  52315 MW;  539501B4671659F3 CRC64;
     MCYNMDMTNF KKNDIFEAEV LDLTHEGQGV VKIDSFPFFV DNALPGERIK MHVLKVGKSF
     GFGRVDEFIS QSKHRVTQLN LDYLRTGIAD FGHLSYDEQV KFKHKQVVDI LRKTAGKSDY
     PVLPVVEAIK TINYRNKAQI PVQMVDGLLT TGFYRKNSHT LIPVEDFYIQ HPEIDAVVLF
     LRDEFRKINL QAYDEKTRKG WLRNIVVRRA FHTGEMMITL VVTSKKLPEK VELVIDHLVE
     RFTNIKSVQL NINSGTGSFI LGKEFILLYG KDFITDLMLD KTYQISAPAF YQVNTPQAEK
     LYETAYEFAG LKPGDVVIDA YSGIGTIGIS MADRVAKVYG MEVVPAAVEN AKRNAQLNEL
     ENTHYEVGTA EKIMPKWLSE GIKPDVIFVD PPRKGLDEGF IKAAAMMNPR RIVYISCNPA
     TFARDVVRFE ADGYLLDKVQ PVDLFPQTHH IELVASFNKK