ID   YLXH_BACSU              Reviewed;         298 AA.
AC   P40742;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Flagellum site-determining protein YlxH;
GN   Name=ylxH; OrderedLocusNames=BSU16410;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7987014; DOI=10.3109/10425179409020852;
RA   Kirsch M.L., Carpenter P.B., Ordal G.W.;
RT   "A putative ATP-binding protein from the che/fla locus of Bacillus
RT   subtilis.";
RL   DNA Seq. 4:271-275(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (3.06 ANGSTROMS) OF 1-23 IN COMPLEX WITH FLHF,
RP   FUNCTION, SUBUNIT, AND MUTAGENESIS OF GLN-8.
RC   STRAIN=168;
RX   PubMed=22056770; DOI=10.1038/nsmb.2141;
RA   Bange G., Kummerer N., Grudnik P., Lindner R., Petzold G., Kressler D.,
RA   Hurt E., Wild K., Sinning I.;
RT   "Structural basis for the molecular evolution of SRP-GTPase activation by
RT   protein.";
RL   Nat. Struct. Mol. Biol. 18:1376-1380(2011).
CC   -!- FUNCTION: Involved in the placement and assembly of flagella (By
CC       similarity). Activates the SRP-GTPase activity of FlhF. {ECO:0000250,
CC       ECO:0000269|PubMed:22056770}.
CC   -!- SUBUNIT: Interacts (via N-terminal region) with GTP-bound FlhF
CC       homodimer (via NG domain). {ECO:0000269|PubMed:22056770}.
CC   -!- INTERACTION:
CC       P40742; Q01960: flhF; NbExp=9; IntAct=EBI-15950871, EBI-15652472;
CC   -!- SIMILARITY: Belongs to the ParA family. {ECO:0000305}.
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DR   EMBL; X73682; CAA52034.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB13514.1; -; Genomic_DNA.
DR   PIR; I40434; I40434.
DR   RefSeq; NP_389523.1; NC_000964.3.
DR   RefSeq; WP_010886507.1; NZ_JNCM01000035.1.
DR   PDB; 3SYN; X-ray; 3.06 A; E/F/G/H=1-23.
DR   PDBsum; 3SYN; -.
DR   AlphaFoldDB; P40742; -.
DR   SMR; P40742; -.
DR   DIP; DIP-59442N; -.
DR   IntAct; P40742; 1.
DR   STRING; 224308.BSU16410; -.
DR   PaxDb; P40742; -.
DR   PRIDE; P40742; -.
DR   EnsemblBacteria; CAB13514; CAB13514; BSU_16410.
DR   GeneID; 940123; -.
DR   KEGG; bsu:BSU16410; -.
DR   PATRIC; fig|224308.43.peg.1736; -.
DR   eggNOG; COG0455; Bacteria.
DR   InParanoid; P40742; -.
DR   OMA; FKVAKNN; -.
DR   PhylomeDB; P40742; -.
DR   BioCyc; BSUB:BSU16410-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0009898; C:cytoplasmic side of plasma membrane; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR   GO; GO:0044781; P:bacterial-type flagellum organization; IEA:UniProtKB-KW.
DR   GO; GO:0051782; P:negative regulation of cell division; IBA:GO_Central.
DR   CDD; cd02038; FlhG-like; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR033875; FlhG.
DR   InterPro; IPR025501; MinD_FleN.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR033756; YlxH/NBP35.
DR   Pfam; PF10609; ParA; 1.
DR   PIRSF; PIRSF003092; MinD; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Bacterial flagellum biogenesis;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..298
FT                   /note="Flagellum site-determining protein YlxH"
FT                   /id="PRO_0000049627"
FT   BINDING         39..46
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         8
FT                   /note="Q->A: Does not affect binding to FlhF, but abolishes
FT                   its activation."
FT                   /evidence="ECO:0000269|PubMed:22056770"
FT   HELIX           10..20
FT                   /evidence="ECO:0007829|PDB:3SYN"
SQ   SEQUENCE   298 AA;  33161 MW;  AF6FE71FA3C1C972 CRC64;
     MQMNRYDQAA TLRAKMEKRE RVLPMVYSQK AKTLAVISGK GGVGKSNITL NMALALQDKG
     KKVLLIDLDI GMGNIDILIG NSSSATIIDV LTDRKPLLQS LSVGPKGLRY ISGGTGLDVM
     FQLDQRKWTF FANELSHALS QFDYVLFDMG AGLSKDQLPF ILSAEDILII TTPEPTAIMD
     AYSAVKHLVL TENKLSMKVA VNRCRDQKEG LDAFARLSRT IHMFLDVQVQ FAGSVSDDVI
     VSKAVVEQVP FFIKSPQAKA SRSVRILADA LFEREETRHK EDKQTFIEKL SSFLMRRA