YM084_YEAST
ID YM084_YEAST Reviewed; 262 AA.
AC A2P2R3; D6VZQ7; E9P8R5; Q3E7C0; Q6B308; Q6WV01;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing];
DE Short=GFAT;
DE EC=2.6.1.16;
DE AltName: Full=D-fructose-6-phosphate amidotransferase;
DE AltName: Full=Hexosephosphate aminotransferase;
GN OrderedLocusNames=YMR084W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 246-262, AND CONFIRMATION OF
RP FRAMESHIFT.
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=12844361; DOI=10.1186/gb-2003-4-7-r45;
RA Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A.,
RA Gates K., Gaffney T.D., Philippsen P.;
RT "Reinvestigation of the Saccharomyces cerevisiae genome annotation by
RT comparison to the genome of a related fungus: Ashbya gossypii.";
RL Genome Biol. 4:R45.1-R45.13(2003).
CC -!- FUNCTION: Involved in amino sugar synthesis (formation of chitin,
CC supplies the amino sugars of asparagine-linked oligosaccharides of
CC glycoproteins). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-
CC phosphate + L-glutamate; Xref=Rhea:RHEA:13237, ChEBI:CHEBI:29985,
CC ChEBI:CHEBI:58359, ChEBI:CHEBI:58725, ChEBI:CHEBI:61527; EC=2.6.1.16;
CC -!- PATHWAY: Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-
CC glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-
CC fructose 6-phosphate: step 1/1.
CC -!- CAUTION: This is a truncated version of a putative glutamine--fructose-
CC 6-phosphate aminotransferase. Strain S288c has a frameshift in position
CC 258, which disrupts the gene coding for this protein and produces two
CC ORFs YMR084W and YMR085W. A contiguous sequence for this protein can be
CC found in strain YJM789 (AC A6ZME2). {ECO:0000305}.
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DR EMBL; Z49259; CAA89230.1; -; Genomic_DNA.
DR EMBL; AY692572; AAT92591.1; -; Genomic_DNA.
DR EMBL; AY268137; AAR26283.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09981.1; -; Genomic_DNA.
DR PIR; S54459; S54459.
DR RefSeq; NP_013801.1; NM_001182583.1.
DR AlphaFoldDB; A2P2R3; -.
DR SMR; A2P2R3; -.
DR BioGRID; 35259; 79.
DR STRING; 4932.YMR084W; -.
DR PaxDb; A2P2R3; -.
DR PRIDE; A2P2R3; -.
DR EnsemblFungi; YMR084W_mRNA; YMR084W; YMR084W.
DR GeneID; 855108; -.
DR KEGG; sce:YMR084W; -.
DR SGD; S000004689; YMR084W.
DR VEuPathDB; FungiDB:YMR084W; -.
DR eggNOG; KOG1268; Eukaryota.
DR GeneTree; ENSGT00940000173234; -.
DR HOGENOM; CLU_012520_6_2_1; -.
DR InParanoid; A2P2R3; -.
DR BioCyc; YEAST:G3O-32784-MON; -.
DR UniPathway; UPA00113; UER00528.
DR PRO; PR:A2P2R3; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; A2P2R3; protein.
DR GO; GO:0004360; F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity; IBA:GO_Central.
DR GO; GO:0006002; P:fructose 6-phosphate metabolic process; IBA:GO_Central.
DR GO; GO:0034221; P:fungal-type cell wall chitin biosynthetic process; IBA:GO_Central.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006487; P:protein N-linked glycosylation; IBA:GO_Central.
DR GO; GO:0006048; P:UDP-N-acetylglucosamine biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006047; P:UDP-N-acetylglucosamine metabolic process; IBA:GO_Central.
DR Gene3D; 3.60.20.10; -; 1.
DR InterPro; IPR017932; GATase_2_dom.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR SUPFAM; SSF56235; SSF56235; 1.
DR PROSITE; PS51278; GATASE_TYPE_2; 1.
PE 3: Inferred from homology;
KW Aminotransferase; Glutamine amidotransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..262
FT /note="Putative glutamine--fructose-6-phosphate
FT aminotransferase [isomerizing]"
FT /id="PRO_0000269757"
FT DOMAIN 2..262
FT /note="Glutamine amidotransferase type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00609"
FT ACT_SITE 2
FT /note="Nucleophile; for GATase activity"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00609"
SQ SEQUENCE 262 AA; 29272 MW; CAEC5457BEB33DFD CRC64;
MCGIFGYCNF LIEKTRGEII DTLIEGLQAL EYKEYDSSGI SIQGDELESL NIYKQTGKIS
SLKEEIDLYN LNKNLPFISH CGIAHTRRAT HGGLRRANCH PHNSDPSNEF VVVHNGVITN
FANLKALLMA KGYVFKSDTD TECIPKLYKH IYDTSIELGY NLDFHVLTNL VLKELEGSYG
LLCTSSHFPD EVVAARKGSP LVIGVKGKTD MDVNFVEVEY LDQEEDYLKL NTQTKSSGNV
LAAAPVKYNT CLRKSPPFVH NT
