YM30_YEAST
ID YM30_YEAST Reviewed; 547 AA.
AC Q03795; D6VZX6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Uncharacterized membrane protein YMR155W;
GN OrderedLocusNames=YMR155W; ORFNames=YM8520.04;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-237, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z49705; CAA89791.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10050.1; -; Genomic_DNA.
DR PIR; S54513; S54513.
DR RefSeq; NP_013876.1; NM_001182658.1.
DR AlphaFoldDB; Q03795; -.
DR BioGRID; 35330; 228.
DR DIP; DIP-4428N; -.
DR IntAct; Q03795; 5.
DR MINT; Q03795; -.
DR STRING; 4932.YMR155W; -.
DR iPTMnet; Q03795; -.
DR MaxQB; Q03795; -.
DR PaxDb; Q03795; -.
DR PRIDE; Q03795; -.
DR EnsemblFungi; YMR155W_mRNA; YMR155W; YMR155W.
DR GeneID; 855187; -.
DR KEGG; sce:YMR155W; -.
DR SGD; S000004764; YMR155W.
DR VEuPathDB; FungiDB:YMR155W; -.
DR eggNOG; ENOG502QQN9; Eukaryota.
DR HOGENOM; CLU_012596_0_1_1; -.
DR InParanoid; Q03795; -.
DR OMA; CLGVDCF; -.
DR BioCyc; YEAST:G3O-32845-MON; -.
DR PRO; PR:Q03795; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q03795; protein.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR010658; Nodulin-like.
DR Pfam; PF06813; Nodulin-like; 1.
DR SUPFAM; SSF103473; SSF103473; 2.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..547
FT /note="Uncharacterized membrane protein YMR155W"
FT /id="PRO_0000203311"
FT TOPO_DOM 1..21
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..58
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..83
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 105..110
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 132..144
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 166..175
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..323
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 324..344
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 345..398
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..419
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 420..437
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 438..458
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 459..469
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 470..490
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 491..514
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 515..535
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 536..547
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 275..300
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 237
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 547 AA; 60044 MW; 63B74B8359920A6D CRC64;
MVKKHQNSKM GNTNHFGHLK SFVGGNVVAL GAGTPYLFSF YAPQLLSKCH IPVSASSKLS
FSLTIGSSLM GILAGIVVDR SPKLSCLIGS MCVFIAYLIL NLCYKHEWSS TFLISLSLVL
IGYGSVSGFY ASVKCANTNF PQHRGTAGAF PVSLYGLSGM VFSYLCSKLF GENIEHVFIF
LMVACGCMIL VGYFSLDIFS NAEGDDASIK EWELQKSRET DDNIVPLYEN SNDYIGSPVR
SSSPATYETY ALSDNFQETS EFFALEDRQL SNRPLLSPSS PHTKYDFEDE NTSKNTVGEN
SAQKSMRLHV FQSLKSSTFI GYYIVLGILQ GVGLMYIYSV GFMVQAQVST PPLNQLPINA
EKIQSLQVTL LSLLSFCGRL SSGPISDFLV KKFKAQRLWN IVIASLLVFL ASNKISHDFS
SIEDPSLRAS KSFKNISVCS AIFGYSFGVL FGTFPSIVAD RFGTNGYSTL WGVLTTGGVF
SVSVFTDILG RDFKANTGDD DGNCKKGVLC YSYTFMVTKY CAAFNLLFVL GIIGYTYYRR
RATANSL
