CBL_ECOLI
ID CBL_ECOLI Reviewed; 316 AA.
AC Q47083; P76353;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=HTH-type transcriptional regulator cbl;
GN Name=cbl; OrderedLocusNames=b1987, JW1966;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8529872; DOI=10.1016/0378-1119(95)00606-8;
RA Iwanicka-Nowicka R., Hryniewicz M.M.;
RT "A new gene, cbl, encoding a member of the LysR family of transcriptional
RT regulators belongs to Escherichia coli cys regulon.";
RL Gene 166:11-17(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: May be an accessory regulatory protein within the cys
CC regulon.
CC -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC {ECO:0000305}.
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DR EMBL; L31639; AAA99929.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75049.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15805.1; -; Genomic_DNA.
DR PIR; C64963; C64963.
DR RefSeq; NP_416492.1; NC_000913.3.
DR RefSeq; WP_001011008.1; NZ_LN832404.1.
DR PDB; 2FYI; X-ray; 2.80 A; A/B/C/D=88-307.
DR PDBsum; 2FYI; -.
DR AlphaFoldDB; Q47083; -.
DR SMR; Q47083; -.
DR BioGRID; 4262113; 6.
DR DIP; DIP-9248N; -.
DR IntAct; Q47083; 2.
DR STRING; 511145.b1987; -.
DR PaxDb; Q47083; -.
DR PRIDE; Q47083; -.
DR EnsemblBacteria; AAC75049; AAC75049; b1987.
DR EnsemblBacteria; BAA15805; BAA15805; BAA15805.
DR GeneID; 946502; -.
DR KEGG; ecj:JW1966; -.
DR KEGG; eco:b1987; -.
DR PATRIC; fig|1411691.4.peg.266; -.
DR EchoBASE; EB4012; -.
DR eggNOG; COG0583; Bacteria.
DR HOGENOM; CLU_039613_6_2_6; -.
DR InParanoid; Q47083; -.
DR OMA; ITYDNAF; -.
DR PhylomeDB; Q47083; -.
DR BioCyc; EcoCyc:G7071-MON; -.
DR EvolutionaryTrace; Q47083; -.
DR PRO; PR:Q47083; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0019344; P:cysteine biosynthetic process; IBA:GO_Central.
DR GO; GO:0045883; P:positive regulation of sulfur utilization; IDA:EcoliWiki.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR CDD; cd08444; PBP2_Cbl; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR037408; Cbl_PBP2.
DR InterPro; IPR005119; LysR_subst-bd.
DR InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00126; HTH_1; 1.
DR Pfam; PF03466; LysR_substrate; 1.
DR PRINTS; PR00039; HTHLYSR.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50931; HTH_LYSR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..316
FT /note="HTH-type transcriptional regulator cbl"
FT /id="PRO_0000105602"
FT DOMAIN 1..59
FT /note="HTH lysR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT DNA_BIND 19..38
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT CONFLICT 143
FT /note="A -> R (in Ref. 1; AAA99929)"
FT /evidence="ECO:0000305"
FT TURN 88..90
FT /evidence="ECO:0007829|PDB:2FYI"
FT STRAND 93..99
FT /evidence="ECO:0007829|PDB:2FYI"
FT HELIX 101..106
FT /evidence="ECO:0007829|PDB:2FYI"
FT HELIX 108..118
FT /evidence="ECO:0007829|PDB:2FYI"
FT STRAND 122..128
FT /evidence="ECO:0007829|PDB:2FYI"
FT HELIX 131..139
FT /evidence="ECO:0007829|PDB:2FYI"
FT STRAND 144..152
FT /evidence="ECO:0007829|PDB:2FYI"
FT STRAND 158..166
FT /evidence="ECO:0007829|PDB:2FYI"
FT STRAND 168..173
FT /evidence="ECO:0007829|PDB:2FYI"
FT HELIX 177..179
FT /evidence="ECO:0007829|PDB:2FYI"
FT STRAND 181..183
FT /evidence="ECO:0007829|PDB:2FYI"
FT HELIX 186..189
FT /evidence="ECO:0007829|PDB:2FYI"
FT STRAND 194..197
FT /evidence="ECO:0007829|PDB:2FYI"
FT HELIX 204..213
FT /evidence="ECO:0007829|PDB:2FYI"
FT STRAND 220..226
FT /evidence="ECO:0007829|PDB:2FYI"
FT HELIX 227..236
FT /evidence="ECO:0007829|PDB:2FYI"
FT STRAND 240..244
FT /evidence="ECO:0007829|PDB:2FYI"
FT HELIX 245..248
FT /evidence="ECO:0007829|PDB:2FYI"
FT STRAND 256..259
FT /evidence="ECO:0007829|PDB:2FYI"
FT TURN 262..264
FT /evidence="ECO:0007829|PDB:2FYI"
FT STRAND 268..275
FT /evidence="ECO:0007829|PDB:2FYI"
FT HELIX 282..291
FT /evidence="ECO:0007829|PDB:2FYI"
FT STRAND 293..295
FT /evidence="ECO:0007829|PDB:2FYI"
FT HELIX 297..305
FT /evidence="ECO:0007829|PDB:2FYI"
SQ SEQUENCE 316 AA; 35856 MW; 70CB59476501067C CRC64;
MNFQQLKIIR EAARQDYNLT EVANMLFTSQ SGVSRHIREL EDELGIEIFV RRGKRLLGMT
EPGKALLVIA ERILNEASNV RRLADLFTND TSGVLTIATT HTQARYSLPE VIKAFRELFP
EVRLELIQGT PQEIATLLQN GEADIGIASE RLSNDPQLVA FPWFRWHHSL LVPHDHPLTQ
ISPLTLESIA KWPLITYRQG ITGRSRIDDA FARKGLLADI VLSAQDSDVI KTYVALGLGI
GLVAEQSSGE QEEENLIRLD TRHLFDANTV WLGLKRGQLQ RNYVWRFLEL CNAGLSVEDI
KRQVMESSEE EIDYQI
