YM54_YEAST
ID YM54_YEAST Reviewed; 1088 AA.
AC Q04336; D6W021;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Uncharacterized protein YMR196W;
GN OrderedLocusNames=YMR196W; ORFNames=YM9646.09;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT "Analysis of phosphorylation sites on proteins from Saccharomyces
RT cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-299; SER-984 AND SER-1081,
RP AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1013, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- MISCELLANEOUS: Present with 2220 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z47815; CAA87818.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10095.1; -; Genomic_DNA.
DR PIR; S50925; S50925.
DR RefSeq; NP_013923.1; NM_001182703.1.
DR AlphaFoldDB; Q04336; -.
DR BioGRID; 35374; 66.
DR DIP; DIP-6550N; -.
DR IntAct; Q04336; 6.
DR MINT; Q04336; -.
DR STRING; 4932.YMR196W; -.
DR iPTMnet; Q04336; -.
DR MaxQB; Q04336; -.
DR PaxDb; Q04336; -.
DR PRIDE; Q04336; -.
DR EnsemblFungi; YMR196W_mRNA; YMR196W; YMR196W.
DR GeneID; 855236; -.
DR KEGG; sce:YMR196W; -.
DR SGD; S000004809; YMR196W.
DR VEuPathDB; FungiDB:YMR196W; -.
DR eggNOG; KOG2161; Eukaryota.
DR GeneTree; ENSGT00390000017452; -.
DR HOGENOM; CLU_005386_0_1_1; -.
DR InParanoid; Q04336; -.
DR OMA; NCRLPQT; -.
DR BioCyc; YEAST:G3O-32883-MON; -.
DR PRO; PR:Q04336; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04336; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0004573; F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity; IEA:InterPro.
DR GO; GO:0009311; P:oligosaccharide metabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR004888; Glycoside_hydrolase_63.
DR PANTHER; PTHR10412; PTHR10412; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..1088
FT /note="Uncharacterized protein YMR196W"
FT /id="PRO_0000203326"
FT REGION 954..980
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1063..1088
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 954..970
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1063..1079
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 299
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 984
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 1013
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 1081
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 1088 AA; 126624 MW; 36C2B42BB2749687 CRC64;
MNKLRDKFVD STVEEERLRE NRNHEKYWYR WGPYLSERSW ATVREDYSLN GDAWSNFPFE
HANARVFRWG EDGLFGVSDN KQLVCMNVAL WNGKDERLKE RLFGLTGPQG NHGEDVKELY
FYLDNTPTHS YMKALYKYPF KKAFPYKELV QKNGERGYED KEFEVYDIDG LYRDSETGDN
PYFDVFFEMA KDDENPSELN FRLTIHNRSK IDSGELYIAP QLFFRNTWAF DGTRTKDKPL
LERDAEAANL INMTHKKYGN CQMVFQPSPG GFSSGTNEEE EDKEVEDIDP LLLFTDNESN
LVKLFNEEKN PSEYTKDAFE EYLVQGKTDA VNPENKGTKA CAVYHFKNIP PGEYVTVRYK
FTNDPKNSIF KAQNLAVVDE DEFDLIFDNR EEEADNFYWR ITPLPISDEL RNLQRQAFSG
LLWTKQFYNF TYDAWYNGDA NVKPRPPPNR ANGRNKNWKH LYIEDILSMP DKWEYPFFAS
WDTAFHCIPL AMIDPEFAKR QLDLLTREWY MHPNGQIPAY EWNFNDVNPP VHAWAVYRVF
KIERNMYNRE DRTFLERVFQ KLLLNFTWWV NRKDTEGKNV FEGGFLGLDN IGVFNRSEPL
PTGGTLEQAD STGWMAFFSL QMLNIALELA KENPVYEDIA SKFFEHFILI SDSMSFEYAT
DITGEKCKEV IKQNLWNEAD KFYYDAISWG DHKVQLPIRS LVGLIPLYAS MTLEPSIIKQ
FRGFKKRVDW FVNNRPEIFD RNIASMSKKG VGERLLLSLV TKERLTAILS RLLDETEFLS
PYGIRSLSKY HEKHPFEMNV NGVEYMVKYL PGESDSGMFG GNSNWRGPIW FPTSFLIMEA
LQRFYLYYGS DFKVECPVGS GDYLNLAEVA EELGYRMIHL FVPDENGERA IHYGDHSKFL
SSDPYFRDYV PFFEYFDGDT GRGLGASHQC GWTALVAKWI SDVGISCVRL PRTPRSSVAT
TASTESSEQG PKMKRMARRK SAKSLVNYTA TILDLTEEEK RHHRIGGTHS GLTPQSSISS
DKARHLMEEM NEEEGIHETV VPEDRHNFET KLIGKLKDKV KNMKVTDKAK DEDIDPMDPM
SPLNKDVS
