YM87_YEAST
ID YM87_YEAST Reviewed; 414 AA.
AC Q04835; D6W080; Q66R30;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Uncharacterized membrane protein YMR253C;
GN OrderedLocusNames=YMR253C; ORFNames=YM9920.07C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-40, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22106047; DOI=10.1002/pmic.201100166;
RA Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL Proteomics 12:236-240(2012).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:14562095}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:14562095}. Note=Localizes to
CC cytoplasmic punctate structures.
CC -!- SIMILARITY: To yeast YPL264c. {ECO:0000305}.
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DR EMBL; Z48639; CAA88580.1; -; Genomic_DNA.
DR EMBL; AY723857; AAU09774.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10154.1; -; Genomic_DNA.
DR PIR; S53075; S53075.
DR RefSeq; NP_013980.1; NM_001182760.1.
DR AlphaFoldDB; Q04835; -.
DR BioGRID; 35432; 34.
DR DIP; DIP-5067N; -.
DR IntAct; Q04835; 4.
DR MINT; Q04835; -.
DR STRING; 4932.YMR253C; -.
DR iPTMnet; Q04835; -.
DR MaxQB; Q04835; -.
DR PaxDb; Q04835; -.
DR PRIDE; Q04835; -.
DR EnsemblFungi; YMR253C_mRNA; YMR253C; YMR253C.
DR GeneID; 855295; -.
DR KEGG; sce:YMR253C; -.
DR SGD; S000004866; YMR253C.
DR VEuPathDB; FungiDB:YMR253C; -.
DR eggNOG; KOG4510; Eukaryota.
DR GeneTree; ENSGT00940000153249; -.
DR HOGENOM; CLU_032828_4_1_1; -.
DR InParanoid; Q04835; -.
DR OMA; VRMFITY; -.
DR BioCyc; YEAST:G3O-32930-MON; -.
DR PRO; PR:Q04835; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04835; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR InterPro; IPR000620; EamA_dom.
DR Pfam; PF00892; EamA; 2.
PE 1: Evidence at protein level;
KW Isopeptide bond; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Ubl conjugation.
FT CHAIN 1..414
FT /note="Uncharacterized membrane protein YMR253C"
FT /id="PRO_0000203340"
FT TOPO_DOM 1..66
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 88..106
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 128..144
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168..171
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..199
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 221..241
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 263..269
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 291..307
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 329..357
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 379..414
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 78..215
FT /note="EamA 1"
FT DOMAIN 253..379
FT /note="EamA 2"
FT REGION 20..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 40
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
FT CONFLICT 278
FT /note="F -> L (in Ref. 3; AAU09774)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 414 AA; 46659 MW; E0EBD0F3BE4CC8AB CRC64;
MNPSVPKVMK RENNTHLLVS KEMNDTSLQL PSTTRSLSPK ESNSNEDFNV DGNETTLQRI
SKDYLKPNIG LVLLTVSYFF NSAMVVSTKV LENDPDDIAN DRQIKPLQIL LVRMVITYIG
TLIYMYINKS TISDVPFGKP EVRKWLVLRG CTGFFGVFGM YYSLMYLTIS DAVLITFLAP
SLTIFLSWVI LRERFTKVEA LGSLISLLGV VLIVRPSFLF GTPELTDSSS QIVESSDPKS
RLIATLVGLW GVLGMSCVYI IIRYIGKRAH AIMSVSYFSL ITAIVSFIGI NTIPSMKFQI
PHSKKQWILF GNLGVSGFIF QLLLTMGIQR ERAGRGSLMT YTQLLYAVFW DVALYKHWPN
IWSWIGMIII ISATLWVIRI RAANNETTAK DLTPIIDDEE NSIPLTEFDL SDSK
