YM8C_YEAST
ID YM8C_YEAST Reviewed; 313 AA.
AC P38430; D6W088; Q6B2J9;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 3.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Uncharacterized deoxyribonuclease YMR262W;
DE EC=3.1.21.-;
GN OrderedLocusNames=YMR262W; ORFNames=YM8156.04;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Manning A.M., Rosenbloom C.L., Beaudet A.L.;
RL Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [3]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 167.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Putative deoxyribonuclease. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250};
CC Note=Binds 2 divalent metal cations per subunit. {ECO:0000250};
CC -!- MISCELLANEOUS: Present with 125 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC TatD-type hydrolase family. {ECO:0000305}.
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DR EMBL; M88172; AAA35223.1; -; Genomic_DNA.
DR EMBL; Z49260; CAA89245.1; -; Genomic_DNA.
DR EMBL; AY692731; AAT92750.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10162.2; -; Genomic_DNA.
DR PIR; S54474; S54474.
DR RefSeq; NP_013989.2; NM_001182769.2.
DR AlphaFoldDB; P38430; -.
DR SMR; P38430; -.
DR BioGRID; 35440; 44.
DR IntAct; P38430; 5.
DR STRING; 4932.YMR262W; -.
DR MaxQB; P38430; -.
DR PaxDb; P38430; -.
DR PRIDE; P38430; -.
DR EnsemblFungi; YMR262W_mRNA; YMR262W; YMR262W.
DR GeneID; 855304; -.
DR KEGG; sce:YMR262W; -.
DR SGD; S000004875; YMR262W.
DR VEuPathDB; FungiDB:YMR262W; -.
DR eggNOG; KOG3020; Eukaryota.
DR GeneTree; ENSGT00720000108846; -.
DR HOGENOM; CLU_031506_3_1_1; -.
DR InParanoid; P38430; -.
DR OMA; PWFSHQI; -.
DR BioCyc; YEAST:G3O-32936-MON; -.
DR PRO; PR:P38430; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P38430; protein.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR InterPro; IPR018228; DNase_TatD-rel_CS.
DR InterPro; IPR032466; Metal_Hydrolase.
DR InterPro; IPR001130; TatD-like.
DR Pfam; PF01026; TatD_DNase; 1.
DR PIRSF; PIRSF005902; DNase_TatD; 1.
DR SUPFAM; SSF51556; SSF51556; 1.
DR PROSITE; PS01091; TATD_3; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Metal-binding; Nuclease; Reference proteome.
FT CHAIN 1..313
FT /note="Uncharacterized deoxyribonuclease YMR262W"
FT /id="PRO_0000203344"
FT BINDING 8
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 10
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 126
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 126
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 180
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 207
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 262
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT CONFLICT 24
FT /note="G -> R (in Ref. 1; AAA35223)"
FT /evidence="ECO:0000305"
FT CONFLICT 167
FT /note="C -> W (in Ref. 2; CAA89245)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 313 AA; 36032 MW; 32FB719DDF5DF9A1 CRC64;
MNKLVDAHCH VITDPDNTFC GDDGGSQGTL RCVMSSNPYD WNNLKKLAGR STSKNDICVG
FGVHPWYSHL FYVGSRRDKV SHYQDVLEYK NEEQFDSLVQ VLPEPLDLEE YIKREFNDTL
VSVIGEIGLD KLFRLPANGF YMQNEKARLT TVKVKLSHQE TVFRRFCRLA RHTSKPISIH
DVKCHGKLND ICNEELLTYH SVKICLHSYT GSKETLLGQW LKKFPPDRIF VSLSKWINFK
DPEEGDALVR SLPSTCILTE TDYPIDNPDP SYQKALTEQL QYLNAQIARA WDETLDASQA
ALRVYENFQK FIK
