YMB8_YEAST
ID YMB8_YEAST Reviewed; 393 AA.
AC Q03730; D6VZF6; Q6B2K7;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Uncharacterized vacuolar membrane protein YML018C;
GN OrderedLocusNames=YML018C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:14562095};
CC Multi-pass membrane protein {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 2010 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. {ECO:0000305}.
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DR EMBL; Z46659; CAA86637.1; -; Genomic_DNA.
DR EMBL; AY692723; AAT92742.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09880.1; -; Genomic_DNA.
DR PIR; S49759; S49759.
DR RefSeq; NP_013694.1; NM_001182376.1.
DR AlphaFoldDB; Q03730; -.
DR SMR; Q03730; -.
DR BioGRID; 35151; 44.
DR DIP; DIP-4953N; -.
DR IntAct; Q03730; 24.
DR MINT; Q03730; -.
DR STRING; 4932.YML018C; -.
DR TCDB; 2.A.7.24.5; the drug/metabolite transporter (dmt) superfamily.
DR iPTMnet; Q03730; -.
DR PaxDb; Q03730; -.
DR PRIDE; Q03730; -.
DR EnsemblFungi; YML018C_mRNA; YML018C; YML018C.
DR GeneID; 854990; -.
DR KEGG; sce:YML018C; -.
DR SGD; S000004480; YML018C.
DR VEuPathDB; FungiDB:YML018C; -.
DR eggNOG; KOG2765; Eukaryota.
DR GeneTree; ENSGT00390000005949; -.
DR HOGENOM; CLU_026578_1_0_1; -.
DR InParanoid; Q03730; -.
DR OMA; KVCRCHK; -.
DR BioCyc; YEAST:G3O-32622-MON; -.
DR PRO; PR:Q03730; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q03730; protein.
DR GO; GO:0000329; C:fungal-type vacuole membrane; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Vacuole.
FT CHAIN 1..393
FT /note="Uncharacterized vacuolar membrane protein YML018C"
FT /id="PRO_0000203261"
FT TOPO_DOM 1..17
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 39..46
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..132
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..156
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 177..182
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..219
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..257
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 279..292
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 314..321
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 322..342
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 343..345
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 346..366
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 367..393
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 93
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT CONFLICT 257
FT /note="K -> R (in Ref. 3; AAT92742)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 393 AA; 43749 MW; DEC5C3EEF293F5ED CRC64;
MVSKDQTSFN KRWTLGLLML GLVIILWVLS SFLINLIFED DSYRKPFFIT YTNTAAFIFY
LFPTAKAVVV NYKDTGRANV HRELIMEEEG TGSDSNRSVD MTSPLLTNLE AGTHANQKKR
LTLYETIKLS AEFCILWFTA NLVTNASLAF TSVASQTILS TTSSFFTLFI GAICHVESLS
KSKVLGSFIS FVGIIMVTKS DSHQRYQRHI ADVSGDDNDA VQVLIGNLLA LAGAVLYGVY
STLLKREVGD ETRVNMKIFF GFVGLFNLLF LWPSLIVLDF FGWEPFSLPK DPKVVVIIFV
NCLITFVSDF CWAKAMLLTS PLTVTVGLSI TIPLAMFGDV IFKHKTMSAL YLFGATLILG
SFFIINKSSE EEHFENSITA SNYESVEVPA ANN
