YMD8_YEAST
ID YMD8_YEAST Reviewed; 442 AA.
AC Q03697; D6VZD7;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Putative nucleotide-sugar transporter YMD8;
GN Name=YMD8; OrderedLocusNames=YML038C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-209, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:14562095}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:14562095}. Cytoplasmic vesicle, COPI-coated vesicle
CC membrane {ECO:0000269|PubMed:14562095}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:14562095}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. SLC35C subfamily.
CC {ECO:0000305}.
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DR EMBL; Z46659; CAA86617.1; -; Genomic_DNA.
DR EMBL; AY692787; AAT92806.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09861.1; -; Genomic_DNA.
DR PIR; S49741; S49741.
DR RefSeq; NP_013674.1; NM_001182396.1.
DR AlphaFoldDB; Q03697; -.
DR BioGRID; 35132; 132.
DR DIP; DIP-1312N; -.
DR IntAct; Q03697; 10.
DR MINT; Q03697; -.
DR STRING; 4932.YML038C; -.
DR TCDB; 2.A.7.9.14; the drug/metabolite transporter (dmt) superfamily.
DR iPTMnet; Q03697; -.
DR MaxQB; Q03697; -.
DR PaxDb; Q03697; -.
DR PRIDE; Q03697; -.
DR EnsemblFungi; YML038C_mRNA; YML038C; YML038C.
DR GeneID; 854970; -.
DR KEGG; sce:YML038C; -.
DR SGD; S000004502; YMD8.
DR VEuPathDB; FungiDB:YML038C; -.
DR eggNOG; KOG1443; Eukaryota.
DR GeneTree; ENSGT00510000048078; -.
DR HOGENOM; CLU_022332_1_1_1; -.
DR InParanoid; Q03697; -.
DR OMA; WYNYYRY; -.
DR BioCyc; YEAST:G3O-32637-MON; -.
DR PRO; PR:Q03697; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q03697; protein.
DR GO; GO:0005801; C:cis-Golgi network; IBA:GO_Central.
DR GO; GO:0030663; C:COPI-coated vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0005338; F:nucleotide-sugar transmembrane transporter activity; ISS:SGD.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0015780; P:nucleotide-sugar transmembrane transport; ISS:SGD.
DR GO; GO:0015786; P:UDP-glucose transmembrane transport; IMP:SGD.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR Pfam; PF03151; TPT; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Glycoprotein; Golgi apparatus; Membrane;
KW Phosphoprotein; Reference proteome; Sugar transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..442
FT /note="Putative nucleotide-sugar transporter YMD8"
FT /id="PRO_0000213402"
FT TOPO_DOM 1..3
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 25..32
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..76
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..107
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 129..132
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..166
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..254
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..301
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 302..322
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 323..329
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 351..355
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 377..442
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 209
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT CARBOHYD 99
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 442 AA; 49615 MW; 37A03543D3AB695E CRC64;
MNRTVFLAFV FGWYFCSIAL SIYNRWMFDP KDGLGIGYPV LVTTFHQATL WLLSGIYIKL
RHKPVKNVLR KNNGFNWSFF LKFLLPTAVA SAGDIGLSNV SFQYVPLTIY TIIKSSSIAF
VLLFGCIFKL EKFHWKLALS VIIMFVGVAL MVFKPSDSTS TKNDQALVIF GSFLVLASSC
LSGLRWVYTQ LMLRNNPIQT NTAAAVEESD GALFTENEDN VDNEPVVNLA NNKMLENFGE
SKPHPIHTIH QLAPIMGITL LLTSLLVEKP FPGIFSSSIF RLDTSNGGVG TETTVLSIVR
GIVLLILPGF AVFLLTICEF SILEQTPVLT VSIVGIVKEL LTVIFGIIIL SERLSGFYNW
LGMLIIMADV CYYNYFRYKQ DLLQKYHSVS TQDNRNELKG FQDFEQLGSK KIAPYSISVD
LTNQEYELDM IAQNVSRSSQ QV
