ID   CBNB_CUPNE              Reviewed;         370 AA.
AC   P83763; Q93T14; Q9WXC9;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Chloromuconate cycloisomerase CbnB;
DE            EC=5.5.1.7;
DE   AltName: Full=Muconate cycloisomerase II CbnB;
GN   Name=cbnB {ECO:0000303|PubMed:9925607};
OS   Cupriavidus necator (Alcaligenes eutrophus) (Ralstonia eutropha).
OG   Plasmid pENH91.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=106590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NH9 {ECO:0000269|PubMed:9925607};
RX   PubMed=9925607; DOI=10.1128/aem.65.2.724-731.1999;
RA   Ogawa N., Miyashita K.;
RT   "The chlorocatechol-catabolic transposon Tn5707 of Alcaligenes eutrophus
RT   NH9, carrying a gene cluster highly homologous to that in the 1,2,4-
RT   trichlorobenzene-degrading bacterium Pseudomonas sp. strain P51, confers
RT   the ability to grow on 3-chlorobenzoate.";
RL   Appl. Environ. Microbiol. 65:724-731(1999).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R)-2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-
CC         cis,cis-muconate + H(+); Xref=Rhea:RHEA:11032, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17589, ChEBI:CHEBI:85538; EC=5.5.1.7;
CC         Evidence={ECO:0000305};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- PATHWAY: Aromatic compound metabolism; 3-chlorocatechol degradation.
CC   -!- SIMILARITY: Belongs to the mandelate racemase/muconate lactonizing
CC       enzyme family. {ECO:0000305}.
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DR   EMBL; AB019032; BAA74531.1; -; Genomic_DNA.
DR   RefSeq; WP_011255151.1; NZ_CP017760.1.
DR   AlphaFoldDB; P83763; -.
DR   SMR; P83763; -.
DR   UniPathway; UPA00083; -.
DR   GO; GO:0018850; F:chloromuconate cycloisomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR   GO; GO:0018849; F:muconate cycloisomerase activity; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0009063; P:cellular amino acid catabolic process; IEA:InterPro.
DR   CDD; cd03318; MLE; 1.
DR   Gene3D; 3.20.20.120; -; 1.
DR   Gene3D; 3.30.390.10; -; 1.
DR   InterPro; IPR013370; Chloromuconate_cycloisomerase.
DR   InterPro; IPR036849; Enolase-like_C_sf.
DR   InterPro; IPR029017; Enolase-like_N.
DR   InterPro; IPR029065; Enolase_C-like.
DR   InterPro; IPR018110; Mandel_Rmase/mucon_lact_enz_CS.
DR   InterPro; IPR013342; Mandelate_racemase_C.
DR   InterPro; IPR013341; Mandelate_racemase_N_dom.
DR   Pfam; PF13378; MR_MLE_C; 1.
DR   Pfam; PF02746; MR_MLE_N; 1.
DR   SFLD; SFLDG01258; (chloro)muconate_cycloisomeras; 1.
DR   SMART; SM00922; MR_MLE; 1.
DR   SUPFAM; SSF51604; SSF51604; 1.
DR   SUPFAM; SSF54826; SSF54826; 1.
DR   TIGRFAMs; TIGR02534; mucon_cyclo; 1.
DR   PROSITE; PS00908; MR_MLE_1; 1.
DR   PROSITE; PS00909; MR_MLE_2; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Isomerase; Manganese; Metal-binding;
KW   Plasmid.
FT   CHAIN           1..370
FT                   /note="Chloromuconate cycloisomerase CbnB"
FT                   /id="PRO_0000171255"
FT   ACT_SITE        165
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        323
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         194
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         220
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         245
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   370 AA;  39465 MW;  195D3101CA18B31C CRC64;
     MKIEAISTTI VDVPTRRPLQ MSFTTVHKQS YVIVQVTAGG LVGIGEGGSV GGPTWGSESA
     ETIKVIIDNY LAPLLIGKDA SNLSEARALM DRAVTGNLSA KAAIDIALHD LKARALNLSI
     ADLIGGTMRK SIPIAWTLAS GDTARDIDSA LEMIEARRHN RFKVKLGART PAQDLEHIRS
     IVKAVGDKAS VRVDVNQGWD EQTASIWIPR LEEAGVELVE QPVPRANFGA LRRLTEQNGV
     AILADESLSS LSSAFELARD RAVDAFSLKL CNMGGIANTL KVAAIAEAAG ISSYGGTMLD
     STVGTAAALH VYATLPSLPY GCELIGPWVL SDRLTQQDLE IKDFEVHLPV GSGLGVDLDH
     DKVRHYTRAA