YMW4_YEAST
ID YMW4_YEAST Reviewed; 145 AA.
AC Q04773; D6VZP8;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Uncharacterized protein YMR074C;
GN OrderedLocusNames=YMR074C; ORFNames=YM9916.13C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-121 AND SER-126, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-121, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- MISCELLANEOUS: Present with 2550 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the PDCD5 family. {ECO:0000305}.
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DR EMBL; Z48952; CAA88799.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09972.1; -; Genomic_DNA.
DR PIR; S52834; S52834.
DR RefSeq; NP_013790.1; NM_001182572.1.
DR PDB; 2FH0; NMR; -; A=36-116.
DR PDB; 2JXN; NMR; -; A=1-116.
DR PDBsum; 2FH0; -.
DR PDBsum; 2JXN; -.
DR AlphaFoldDB; Q04773; -.
DR BMRB; Q04773; -.
DR SMR; Q04773; -.
DR BioGRID; 35249; 119.
DR DIP; DIP-5242N; -.
DR IntAct; Q04773; 1.
DR MINT; Q04773; -.
DR STRING; 4932.YMR074C; -.
DR iPTMnet; Q04773; -.
DR MaxQB; Q04773; -.
DR PaxDb; Q04773; -.
DR PRIDE; Q04773; -.
DR EnsemblFungi; YMR074C_mRNA; YMR074C; YMR074C.
DR GeneID; 855096; -.
DR KEGG; sce:YMR074C; -.
DR SGD; S000004678; YMR074C.
DR VEuPathDB; FungiDB:YMR074C; -.
DR eggNOG; KOG3431; Eukaryota.
DR GeneTree; ENSGT00390000011085; -.
DR HOGENOM; CLU_122978_0_0_1; -.
DR InParanoid; Q04773; -.
DR OMA; KPQFGEQ; -.
DR BioCyc; YEAST:G3O-32776-MON; -.
DR EvolutionaryTrace; Q04773; -.
DR PRO; PR:Q04773; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04773; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR Gene3D; 1.10.8.140; -; 1.
DR InterPro; IPR002836; PDCD5-like.
DR InterPro; IPR036883; PDCD5-like_sf.
DR PANTHER; PTHR10840; PTHR10840; 1.
DR Pfam; PF01984; dsDNA_bind; 1.
DR PIRSF; PIRSF015730; TFAR19; 1.
DR SUPFAM; SSF46950; SSF46950; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Phosphoprotein; Reference proteome.
FT CHAIN 1..145
FT /note="Uncharacterized protein YMR074C"
FT /id="PRO_0000121548"
FT REGION 15..41
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 125..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..36
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 121
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT MOD_RES 126
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT HELIX 3..15
FT /evidence="ECO:0007829|PDB:2JXN"
FT HELIX 44..47
FT /evidence="ECO:0007829|PDB:2FH0"
FT HELIX 52..64
FT /evidence="ECO:0007829|PDB:2FH0"
FT HELIX 66..82
FT /evidence="ECO:0007829|PDB:2FH0"
FT HELIX 91..104
FT /evidence="ECO:0007829|PDB:2FH0"
SQ SEQUENCE 145 AA; 15977 MW; 36EEA1AFA3F6135D CRC64;
MDPELQAIRE ARLAQLKNNS GGTNGDRNSG ANNGGGENSA PVGAAIANFL EPQALERLSR
VALVRRDRAQ AVETYLKKLI ATNNVTHKIT EAEIVSILNG IAKQQNSQNN SKIIFERKDF
SEDLNSFDKQ NAKNDDDEDD DDFFD
