YMXG_BACSU
ID YMXG_BACSU Reviewed; 409 AA.
AC Q04805;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 3.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Uncharacterized zinc protease YmxG;
DE EC=3.4.24.-;
DE AltName: Full=ORFP;
GN Name=ymxG; OrderedLocusNames=BSU16710;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / 8G5;
RA Bolhuis A., Vehmaanpera J., Venema G., Bron S., van Dijl J.M.;
RL Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 8.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-409.
RC STRAIN=168;
RX PubMed=8098035; DOI=10.1016/s0021-9258(18)98372-9;
RA Chen N.-Y., Jiang S.-Q., Klein D.A., Paulus H.;
RT "Organization and nucleotide sequence of the Bacillus subtilis
RT diaminopimelate operon, a cluster of genes encoding the first three enzymes
RT of diaminopimelate synthesis and dipicolinate synthase.";
RL J. Biol. Chem. 268:9448-9465(1993).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- SIMILARITY: Belongs to the peptidase M16 family. {ECO:0000305}.
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DR EMBL; U27560; AAA73485.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13544.2; -; Genomic_DNA.
DR EMBL; L08471; AAA22379.1; -; Genomic_DNA.
DR PIR; E69886; E69886.
DR RefSeq; NP_389553.2; NC_000964.3.
DR RefSeq; WP_003245717.1; NZ_CP053102.1.
DR AlphaFoldDB; Q04805; -.
DR SMR; Q04805; -.
DR STRING; 224308.BSU16710; -.
DR MEROPS; M16.A15; -.
DR PaxDb; Q04805; -.
DR PRIDE; Q04805; -.
DR EnsemblBacteria; CAB13544; CAB13544; BSU_16710.
DR GeneID; 938456; -.
DR KEGG; bsu:BSU16710; -.
DR PATRIC; fig|224308.179.peg.1812; -.
DR eggNOG; COG0612; Bacteria.
DR InParanoid; Q04805; -.
DR OMA; IDVVCDM; -.
DR PhylomeDB; Q04805; -.
DR BioCyc; BSUB:BSU16710-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR InterPro; IPR011249; Metalloenz_LuxS/M16.
DR InterPro; IPR011765; Pept_M16_N.
DR InterPro; IPR001431; Pept_M16_Zn_BS.
DR InterPro; IPR007863; Peptidase_M16_C.
DR Pfam; PF00675; Peptidase_M16; 1.
DR Pfam; PF05193; Peptidase_M16_C; 1.
DR SUPFAM; SSF63411; SSF63411; 2.
DR PROSITE; PS00143; INSULINASE; 1.
PE 3: Inferred from homology;
KW Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome;
KW Zinc.
FT CHAIN 1..409
FT /note="Uncharacterized zinc protease YmxG"
FT /id="PRO_0000074420"
FT ACT_SITE 49
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT BINDING 46
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT BINDING 50
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT BINDING 126
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT CONFLICT 8
FT /note="Q -> P (in Ref. 1; AAA73485)"
FT /evidence="ECO:0000305"
FT CONFLICT 270..271
FT /note="FQ -> LE (in Ref. 4; AAA22379)"
FT /evidence="ECO:0000305"
FT CONFLICT 360
FT /note="E -> Q (in Ref. 4; AAA22379)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 409 AA; 45995 MW; E5C45B3E85B40509 CRC64;
MIKRYTCQNG VRIVLENNPT VRSVAIGVWI GTGSRHETPE INGISHFLEH MFFKGTSTKS
AREIAESFDR IGGQVNAFTS KEYTCYYAKV LDEHANYALD VLADMFFHST FDENELKKEK
NVVYEEIKMY EDAPDDIVHD LLSKATYGNH SLGYPILGTE ETLASFNGDS LRQYMHDYYT
PDRVVISVAG NISDSFIKDV EKWFGSYEAK GKATGLEKPE FHTEKLTRKK ETEQAHLCLG
FKGLEVGHER IYDLIVLNNV LGGSMSSRLF QDVREDKGLA YSVYSYHSSY EDSGMLTIYG
GTGANQLQQL SETIQETLAT LKRDGITSKE LENSKEQMKG SLMLSLESTN SKMSRNGKNE
LLLGKHKTLD EIINELNAVN LERVNGLARQ LFTEDYALAL ISPSGNMPS
