CBP4A_DICDI
ID CBP4A_DICDI Reviewed; 162 AA.
AC Q54RF4; Q9GYE0;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Calcium-binding protein 4a;
GN Name=cbpD1 {ECO:0000312|EMBL:EAL65815.1}; ORFNames=DDB_G0283153;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1] {ECO:0000312|EMBL:CAC04145.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=AX2 {ECO:0000312|EMBL:CAC04145.1};
RX PubMed=10771103; DOI=10.1016/s0167-4889(00)00024-0;
RA Dorywalska M., Coukell B., Dharamsi A.;
RT "Characterization and hetereologous expression of cDNAs encoding two novel
RT closely related Ca(2+)-binding proteins in Dictyostelium discoideum.";
RL Biochim. Biophys. Acta 1496:356-361(2000).
RN [2] {ECO:0000312|EMBL:EAL65815.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4 {ECO:0000312|EMBL:EAL65815.1};
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3] {ECO:0000305}
RP INTERACTION WITH NUMA.
RX PubMed=15325281; DOI=10.1016/j.bbrc.2004.07.168;
RA Myre M.A., O'Day D.H.;
RT "Dictyostelium calcium-binding protein 4a interacts with nucleomorphin, a
RT BRCT-domain protein that regulates nuclear number.";
RL Biochem. Biophys. Res. Commun. 322:665-671(2004).
RN [4] {ECO:0000305}
RP DEVELOPMENTAL STAGE.
RX PubMed=11923193; DOI=10.1242/dev.129.7.1543;
RA Van Driessche N., Shaw C., Katoh M., Morio T., Sucgang R., Ibarra M.,
RA Kuwayama H., Saito T., Urushihara H., Maeda M., Takeuchi I., Ochiai H.,
RA Eaton W., Tollett J., Halter J., Kuspa A., Tanaka Y., Shaulsky G.;
RT "A transcriptional profile of multicellular development in Dictyostelium
RT discoideum.";
RL Development 129:1543-1552(2002).
RN [5] {ECO:0000305}
RP DEVELOPMENTAL STAGE.
RX PubMed=12912885; DOI=10.1128/ec.2.4.664-670.2003;
RA Iranfar N., Fuller D., Loomis W.F.;
RT "Genome-wide expression analyses of gene regulation during early
RT development of Dictyostelium discoideum.";
RL Eukaryot. Cell 2:664-670(2003).
CC -!- SUBUNIT: Interacts with numA/nucleomorphin in the presence of Ca(2+).
CC {ECO:0000269|PubMed:15325281}.
CC -!- INTERACTION:
CC Q54RF4; Q9U7C9: numA; NbExp=2; IntAct=EBI-531591, EBI-530708;
CC -!- DEVELOPMENTAL STAGE: Expressed during development. Expression levels
CC increase steadily during aggregation and peak at 10 hours until after
CC which they decrease steadily until late culmination.
CC {ECO:0000269|PubMed:11923193, ECO:0000269|PubMed:12912885}.
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DR EMBL; AJ297597; CAC04145.1; -; mRNA.
DR EMBL; AAFI02000051; EAL65815.1; -; Genomic_DNA.
DR RefSeq; XP_639191.1; XM_634099.1.
DR AlphaFoldDB; Q54RF4; -.
DR SMR; Q54RF4; -.
DR IntAct; Q54RF4; 1.
DR STRING; 44689.DDB0219925; -.
DR PaxDb; Q54RF4; -.
DR EnsemblProtists; EAL65815; EAL65815; DDB_G0283153.
DR GeneID; 8623967; -.
DR KEGG; ddi:DDB_G0283153; -.
DR dictyBase; DDB_G0283153; cbpD1.
DR HOGENOM; CLU_1638493_0_0_1; -.
DR InParanoid; Q54RF4; -.
DR PhylomeDB; Q54RF4; -.
DR PRO; PR:Q54RF4; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IDA:dictyBase.
DR GO; GO:0005654; C:nucleoplasm; IDA:dictyBase.
DR GO; GO:0005509; F:calcium ion binding; IDA:dictyBase.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13202; EF-hand_5; 2.
DR SMART; SM00054; EFh; 4.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 3.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 1: Evidence at protein level;
KW Calcium; Metal-binding; Reference proteome; Repeat.
FT CHAIN 1..162
FT /note="Calcium-binding protein 4a"
FT /id="PRO_0000293631"
FT DOMAIN 10..45
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 46..81
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 85..120
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 123..158
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 23
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 25
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 27
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 29
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 34
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 59
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 61
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 63
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 65
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 70
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 136
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 138
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 140
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 142
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 147
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT CONFLICT 123
FT /note="Q -> P (in Ref. 1; CAC04145)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 162 AA; 18704 MW; CA2391BF26E2145B CRC64;
MVTKKEFLED LTNATNEMIK AGDKNGDNQL SKKELYELFK KCKYPNPTLV TNSLLELFDL
DKDGKLSVNE VKTALLVDYI IEAETCLKKF VDSIFKADSN KDNKITWDEA RQFFVTSGSN
EAQAKVFANS MFEDVDSDDD KCITREELRE YAIEYYEIYP TE
